来自热液喷口虾类——长眼柄铠甲虾（Rimicaris exoculata）的微小RNA特征分析。
Characterization of miRNAs from hydrothermal vent shrimp Rimicaris exoculata.
作者信息
Zhou Yadong, He Yaodong, Wang Chunsheng, Zhang Xiaobo
机构信息
Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China; Key Laboratory of Marine Ecosystem and Biogeochemistry, The Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
出版信息
Mar Genomics. 2015 Dec;24 Pt 3:371-8. doi: 10.1016/j.margen.2015.09.004. Epub 2015 Oct 2.
Deep-sea hydrothermal vent shrimp Rimicaris exoculata is a dominant species aggregating in vent fields along the Mid-Atlantic Ocean Ridge. MicroRNAs play important roles in life cycles of eukaryotes. However, little is known about miRNAs of vent animals. In the present study, a small RNA cDNA library from the muscle of R. exoculata was constructed and the miRNA sequencing was performed. The results indicated that a total of 7,983,331 raw reads were obtained, representing 569,354 unique sequences. Based on sequence analysis, R. exoculata contained 159 conserved miRNAs and 34 novel miRNAs. The conserved miRNAs included 54 families belonging to three different taxonomic units (bilaterian, protostomes and arthropods). The results also showed that miR-2001, a lost miRNA in crustaceans, existed in R. exoculata. Among the conserved miRNAs, iso-miRs were detected. Therefore, this study presented the first insight into the miRNAs of deep-sea hydrothermal vent animals.
深海热液喷口虾类——长腕铠甲虾是聚集在大西洋中脊热液区的优势物种。微小RNA在真核生物的生命周期中发挥着重要作用。然而，关于热液区动物的微小RNA却知之甚少。在本研究中，构建了长腕铠甲虾肌肉的小RNA cDNA文库并进行了微小RNA测序。结果表明，共获得7,983,331条原始读数，代表569,354个独特序列。基于序列分析，长腕铠甲虾包含159个保守微小RNA和34个新的微小RNA。保守微小RNA包括属于三个不同分类单元（两侧对称动物、原口动物和节肢动物）的54个家族。结果还表明，甲壳类动物中已消失的微小RNA——miR-2001存在于长腕铠甲虾中。在保守微小RNA中检测到了异微小RNA。因此，本研究首次揭示了深海热液喷口动物的微小RNA情况。
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