Wang Ruojun, Ehrt Sabine
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, United States.
Front Microbiol. 2021 Apr 20;12:626461. doi: 10.3389/fmicb.2021.626461. eCollection 2021.
Proper control of cell division in the intracellular pathogen is central to its growth, survival, pathogenesis, and resistance to antibiotics. Nevertheless, the divisome components and mechanisms by which mycobacteria regulate their cell cycle are not entirely understood. Here we demonstrate that the previously uncharacterized Rv0954 protein localizes to the mid-cell during cell division and interacts with the division-related proteins LamA, PbpA, and PknH. Deletion of did not result in alterations in cell morphology or sensitivity to cell wall-targeting antibiotics but transposon mutagenesis demonstrated genetic interactions with genes related to cell division. This work suggests that Rv0954 participates in cell division and reveals potential components of the mycobacterial divisome for future investigation.
对细胞内病原体中细胞分裂的适当控制对其生长、存活、致病机制以及对抗生素的抗性至关重要。然而，分枝杆菌调节其细胞周期的分裂体成分和机制尚未完全明确。在此，我们证明了先前未被鉴定的Rv0954蛋白在细胞分裂期间定位于细胞中部，并与与分裂相关的蛋白LamA、PbpA和PknH相互作用。Rv0954的缺失并未导致细胞形态改变或对细胞壁靶向抗生素的敏感性变化，但转座子诱变显示其与细胞分裂相关基因存在遗传相互作用。这项研究表明Rv0954参与细胞分裂，并揭示了分枝杆菌分裂体的潜在组成成分以供未来研究。
