无饲养层的人胚胎干细胞在定义培养条件下的培养。
Feeder-Free Human Embryonic Stem Cell Culture Under Defined Culture Conditions.
机构信息
Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
出版信息
Methods Mol Biol. 2022;2520:25-35. doi: 10.1007/7651_2021_404.
Human embryonic stem (ES) cell culture has developed over the years allowing the subtle procedures that are easy to manipulate. Feeder-free ES cell culture is an important milestone for human pluripotent stem cell research which eliminates the feeder cells. Various matrices and medium formulations have been generated for feeder-independent culture. Here we described an mTeSR™1 based feeder-independent human ES cell culture on Matrigel matrix. Culture, freeze/thaw, passaging and initiation of differentiation in suspension culture were described.
人类胚胎干细胞（ES）培养技术近年来发展迅速，其中一些精细的操作变得更加容易。无饲养层 ES 细胞培养是人类多能干细胞研究的一个重要里程碑，它消除了饲养层细胞。已经生成了各种基质和培养基配方用于无饲养层培养。在这里，我们描述了一种基于 mTeSR™1 的无饲养层人类 ES 细胞在 Matrigel 基质上的培养。描述了细胞培养、冻存/复苏、传代和悬浮培养起始分化。
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