König J J, Hagemeijer A, Smit B, Kamst E, Romijn J C, Schröder F H
Department of Urology, Erasmus University of Rotterdam, The Netherlands.
Cancer Genet Cytogenet. 1988 Aug;34(1):91-9. doi: 10.1016/0165-4608(88)90173-2.
Detailed cytogenetic analysis was performed of a xenografted human prostatic adenocarcinoma cell line PC-82. A direct preparation method was developed that yielded metaphases of good quality. Flow cytometric data and banding analysis of metaphases showed a near-tetraploid karyotype with 18 consistent marker chromosomes. As a result of the rearrangements involved, parts of chromosomes 2, 3, 4, 7, 9, 10, 15, 18, and 21 were homozygous, while regions on 2p, 13q, and 17q were apparently completely lost. In contrast to this, some regions on #2, #5, and, especially, on #1 were present in three or even four times the normal copy number. Comparison of affected chromosomes in PC-82 with all data available on prostatic carcinoma showed chromosomes 1, 2, 3, 6, 7, 10, and 15 to be involved in rearrangements in over 50% of all prostatic carcinomas. When only data from primary prostatic adenocarcinomas (including PC-82) were taken into account it appeared that chromosomes 1, 7, and 10 were involved in all five primary tumors studied.
对异种移植的人前列腺腺癌细胞系PC - 82进行了详细的细胞遗传学分析。开发了一种直接制片方法，可产生高质量的中期分裂相。中期分裂相的流式细胞术数据和显带分析显示为近四倍体核型，有18条一致的标记染色体。由于涉及的重排，染色体2、3、4、7、9、10、15、18和21的部分区域是纯合的，而2p、13q和17q区域明显完全缺失。与此相反，2号、5号染色体上的一些区域，尤其是1号染色体上的一些区域，其拷贝数是正常拷贝数的三倍甚至四倍。将PC - 82中受影响的染色体与前列腺癌的所有可用数据进行比较，发现超过50%的前列腺癌中，染色体1、2、3、6、7、10和15参与了重排。当仅考虑原发性前列腺腺癌（包括PC - 82）的数据时，似乎在所研究的所有五个原发性肿瘤中，染色体1、7和10都参与其中。
