Southern Illinois University School of Dental Medicine, Alton, Illinois, USA.
Clin Exp Dent Res. 2021 Dec;7(6):995-1001. doi: 10.1002/cre2.437. Epub 2021 May 6.
The use of adjunct post-treatment mouth rinses containing chlorhexidine (CHX) for periodontitis patients is associated with side effects that reduce patient compliance. Our aim was to evaluate the proinflammatory and cell proliferation effects of an activated-zinc mouth rinse (SM) that has been suggested as an alternative post-treatment therapeutic.
Tissue models of gingival epithelium were used to simulate periodontal disease and compare inflammatory reactions after treatment with CHX or SM. Tissues were exposed to Porphyromonas gingivalis LPS and wounded to simulate periodontal disease. Tissues were treated and incubated for 6, 12, or 24 h. Inflammatory cytokines were measured in culture medium by ELISA and local expression of Toll-like receptor (TLR)-4 and proliferation marker Ki-67 was visualized by immunohistochemistry.
SM and CHX treatments decreased secretion of IL-1β and IL-8 into culture media at all time points. IL-1β secretion levels were further decreased by SM compared to CHX treatment at all time points. TLR-4 expression appeared significantly increased 12 h post-treatment in the CHX tissues but remained relatively low in SM tissues at all time points. Ki-67 results suggest that cell proliferation was increased in the SM tissues earlier than CHX tissues.
Our data suggest that SM may reduce inflammation in gingival tissues.
使用含有洗必泰（CHX）的辅助治疗后漱口液治疗牙周炎患者会产生副作用，降低患者的依从性。我们的目的是评估一种已被提议作为替代治疗后治疗的活性锌漱口液（SM）的促炎和细胞增殖作用。
使用牙龈上皮组织模型模拟牙周病，并比较 CHX 或 SM 治疗后的炎症反应。组织暴露于牙龈卟啉单胞菌 LPS 并受伤以模拟牙周病。组织经过处理和孵育 6、12 或 24 小时。通过 ELISA 测量培养物中的炎症细胞因子，并通过免疫组织化学可视化 Toll 样受体（TLR）-4 和增殖标志物 Ki-67 的局部表达。
SM 和 CHX 处理在所有时间点均降低了培养物中 IL-1β和 IL-8 的分泌。与 CHX 处理相比，SM 在所有时间点均进一步降低了 IL-1β 的分泌水平。CHX 组织中 TLR-4 表达在治疗后 12 小时明显增加，但在 SM 组织中所有时间点均相对较低。Ki-67 的结果表明，SM 组织中的细胞增殖比 CHX 组织更早增加。
我们的数据表明，SM 可能会减少牙龈组织的炎症。
