Teng Fei, He Tao, Huang Shi, Bo Cun-Pei, Li Zhen, Chang Jin-Lan, Liu Ji-Quan, Charbonneau Duane, Xu Jian, Li Rui, Ling Jun-Qi
Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
Single-Cell Center and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
Int J Oral Sci. 2016 Sep 29;8(3):182-90. doi: 10.1038/ijos.2016.18.
Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride (CPC), can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remains poorly understood. Via a double-blinded, randomised controlled trial of 91 subjects, the impact of CPC-containing oral rinses on supragingival plaque was investigated in experimental gingivitis, where the subjects, after a 21-day period of dental prophylaxis to achieve healthy gingivae, received either CPC rinses or water for 21 days. Within-subject temporal dynamics of plaque microbiota and symptoms of gingivitis were profiled via 16S ribosomal DNA gene pyrosequencing and assessment with the Mazza gingival index. Cetylpyridinium chloride conferred gingival benefits, as progression of gingival inflammation resulting from a lack of dental hygiene was significantly slower in the mouth rinse group than in the water group due to inhibition of 17 gingivitis-enriched bacterial genera. Tracking of plaque α and β diversity revealed that CPC treatment prevents acquisition of new taxa that would otherwise accumulate but maintains the original biodiversity of healthy plaques. Furthermore, CPC rinses reduced the size, local connectivity and microbiota-wide connectivity of the bacterial correlation network, particularly for nodes representing gingivitis-enriched taxa. The findings of this study provide mechanistic insights into the impact of oral rinses on the progression and maturation of dental plaque in the natural human population.
含有化学治疗剂的口腔含漱液，如西吡氯铵（CPC），可以减轻牙菌斑引起的牙龈感染，但口腔微生物群如何在人群中对这些治疗作出反应仍知之甚少。通过一项对91名受试者进行的双盲随机对照试验，研究了含CPC的口腔含漱液在实验性牙龈炎中对龈上菌斑的影响，在该试验中，受试者在经过21天的牙齿预防以达到健康牙龈后，接受CPC含漱液或水，为期21天。通过16S核糖体DNA基因焦磷酸测序和用马扎牙龈指数进行评估，分析了菌斑微生物群的个体内时间动态和牙龈炎症状。西吡氯铵对牙龈有益，因为由于缺乏口腔卫生导致的牙龈炎症进展在含漱液组中比在水组中明显更慢，这是由于17个富含牙龈炎的细菌属受到抑制。对菌斑α和β多样性的追踪表明，CPC治疗可防止新分类群的获得，否则这些分类群会积累，但可维持健康菌斑的原始生物多样性。此外，CPC含漱液减小了细菌相关网络的大小、局部连通性和微生物群范围的连通性，特别是对于代表富含牙龈炎分类群的节点。本研究结果为口腔含漱液对自然人群中牙菌斑进展和成熟的影响提供了机制性见解。
