University of Health Sciences, Sisli Etfal Training and Research Hospital, Turkey.
University of Health Sciences, Sisli Etfal Training and Research Hospital, Turkey.
J Microbiol Methods. 2021 Jun;185:106232. doi: 10.1016/j.mimet.2021.106232. Epub 2021 May 4.
Rapid and accurate diagnosis is critically important in invasive and disseminated fungal infections for appropriate antifungal treatment.
MALDI-TOF MS systems are effective for fast and accurate identification of Candida species.
We aimed to compare two MALDI-TOF MS systems for the rapid identification of non-albicans Candida and rare clinical yeast species.
This study included 157 isolates representing 23 yeast species. All isolates were identified using Bruker MALDI Biotyper and VITEK MS systems. If both MALDI-TOF MS systems yielded the same results for a certain isolate, the identification is regarded as correct. We performed internal transcribed spacer (ITS) DNA sequencing on five fungal isolates with discordant species names or that were unidentified by the two MALDI-TOF MS systems.
The yeast identification sensitivity of MALDI Biotyper was 98.7%, whereas that of VITEK MS was 96.8%. Both MALDI-TOF MS systems correctly identified all strains belonging to four prevalent species, namely, Candida parapsilosis, Candida tropicalis, Candida glabrata, and Candida krusei. For the 19 rare clinical yeast species, identification rates were 96.7% for MALDI Biotyper and 91.7% for VITEK MS. The ITS sequence analysis of five isolates yielded two Meyerozyma caribbica, two Cyberlindnera fabianii, and one Candida dubliniensis.
This study showed the high performance of both MALDI-TOF MS systems, identifying over 90% of yeast isolates in a short time. The disadvantages of these systems are that some species are not present in the databases and it cannot distinguish closely related species. The sensitivity of MALDI-TOF MS systems constantly improves with the expansion of databases in parallel with taxonomic developments for the identification of rare clinical yeast species.
在侵袭性和播散性真菌感染中，快速准确的诊断对于进行适当的抗真菌治疗至关重要。
MALDI-TOF MS 系统可有效快速准确地鉴定念珠菌属物种。
我们旨在比较两种 MALDI-TOF MS 系统，以快速鉴定非白念珠菌属念珠菌和罕见的临床酵母种。
本研究包括 157 株代表 23 种酵母的分离株。所有分离株均使用 Bruker MALDI Biotyper 和 VITEK MS 系统进行鉴定。如果两种 MALDI-TOF MS 系统对同一分离株的鉴定结果相同，则鉴定结果视为正确。我们对 5 株具有不同种名或两种 MALDI-TOF MS 系统均无法鉴定的真菌分离株进行了内部转录间隔区（ITS）DNA 测序。
MALDI Biotyper 的酵母鉴定灵敏度为 98.7%，而 VITEK MS 的灵敏度为 96.8%。两种 MALDI-TOF MS 系统均正确鉴定了属于四个流行种的所有菌株，即近平滑念珠菌、热带念珠菌、光滑念珠菌和克柔念珠菌。对于 19 种罕见的临床酵母种，MALDI Biotyper 的鉴定率为 96.7%，VITEK MS 的鉴定率为 91.7%。对 5 株分离株的 ITS 序列分析得到了两株 M. caribbica、两株 C. fabianii 和一株 C. dubliniensis。
本研究表明两种 MALDI-TOF MS 系统性能良好，可在短时间内鉴定超过 90%的酵母分离株。这些系统的缺点是有些种不在数据库中，也不能区分密切相关的种。随着数据库的扩展和分类学的发展，用于鉴定罕见临床酵母种，MALDI-TOF MS 系统的灵敏度不断提高。
