POLARIS Research Centre, Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126 Milano, Italy,.
Section of Occupational Toxicology, National Institute of Occupational Health, Oslo N-0033, Norway.
Toxicol In Vitro. 2021 Sep;75:105190. doi: 10.1016/j.tiv.2021.105190. Epub 2021 May 5.
Combustion-derived particles (CDPs), in particular from traffic, are regarded as a central contributor for adverse health effects linked to air pollution. Recently, also biomass burning has been recognized as an important source for CDPs. Here, the effects of CDPs (PM) originating from burning of pellet, charcoal and wood on key processes associated to lung carcinogenesis were explored. Human bronchial epithelial cells (HBEC3-KT) were exposed to 2.5 μg/cm of CDPs for 24 h and biological effects were examined in terms of cytotoxicity, inflammation, epithelial to mesenchymal transition (EMT)-related effects, DNA damage and genotoxicity. Reduced cell migration, inflammation and modulation of various PM-associated genes were observed mainly after exposure to wood and pellet. In contrast, only particles from pellet burning induced alteration in cell proliferation and DNA damage, which resulted in cell cycle alterations. Charcoal instead, appeared in general less effective in inducing pro-carcinogenic effects. These results illustrate differences in the toxicological profile due to the CDPs source. The different chemical compounds adsorbed on CDPs seemed to be central for particle properties, leading to an activation of various cellular signaling pathways involved in early steps of cancer progression.
燃烧衍生颗粒（CDP），特别是来自交通的 CDP，被认为是与空气污染有关的不良健康影响的主要因素。最近，生物质燃烧也被认为是 CDP 的一个重要来源。在这里，研究了来自颗粒、木炭和木材燃烧的 CDP（PM）对与肺癌发生相关的关键过程的影响。将人支气管上皮细胞（HBEC3-KT）暴露于 2.5μg/cm 的 CDP 中 24 小时，并检查细胞毒性、炎症、上皮-间充质转化（EMT）相关效应、DNA 损伤和遗传毒性等方面的生物学效应。主要观察到暴露于木材和颗粒后，细胞迁移减少、炎症和各种与 PM 相关的基因发生调节。相比之下，只有来自颗粒燃烧的颗粒才会诱导细胞增殖和 DNA 损伤的改变，从而导致细胞周期改变。而木炭则通常在诱导致癌前效应方面效果较差。这些结果说明了由于 CDP 来源的不同，其毒理学特征也存在差异。吸附在 CDP 上的不同化学化合物似乎是颗粒特性的核心，导致参与癌症进展早期步骤的各种细胞信号通路被激活。
