Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China; School of Stomatology, Qingdao University, Qingdao, 266003, China.
School of Stomatology, Qingdao University, Qingdao, 266003, China; Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266021, China.
Colloids Surf B Biointerfaces. 2021 Aug;204:111802. doi: 10.1016/j.colsurfb.2021.111802. Epub 2021 Apr 29.
Titanium (Ti) is widely applied as bone-anchoring implants in dental and orthopedic applications owing to its superior mechanical characteristics, high corrosion resistance, and excellent biocompatibility. Nevertheless, Ti-based implants with the deficiencies of insufficient osteoinduction and associated infections can result in implant failure, which significantly limits its applications in some cases. In this work, hierarchically hybrid biocoatings on Ti implants are developed by gradual incorporation of polydopamine (PDA), ZnO nanoparticles (nZnO), and chitosan (CS)/nanocrystal hydroxyapatite (nHA) via oxidative self-polymerization, nanoparticle deposition, solvent casting and evaporation methods for enhancing their antibacterial activity and osteogenesis. The modification of PDA on porous reticular Ti substrates greatly reduces the surface roughness, wettability, protein adsorption, and provides high adhesion to the deposited nZnO. Further, incorporating nZnO on PDA-coated Ti surfaces affects the surface structure and wettability, significantly inhibits the growth of both Staphylococcus aureus and Escherichia coli. Moreover, the CS/nHA-doped coating on the nZnO-modified Ti surfaces remarkably improves cytocompatibility and enhances the osteogenic differentiation of MC3T3-E1 cells by upregulating the protein expression of alkaline phosphatase. This work offers a promising alternative for developing Ti implants with long-lifetime bioactivity to achieve strong antibacterial ability and enhanced bone formation for potential dental/orthopedic applications.
钛（Ti）由于其优异的机械性能、高耐腐蚀性和出色的生物相容性，被广泛应用于牙科和骨科领域作为骨锚固植入物。然而，基于 Ti 的植入物存在成骨诱导不足和相关感染的缺陷，这可能导致植入物失败，在某些情况下显著限制了其应用。在这项工作中，通过逐步引入聚多巴胺（PDA）、氧化锌纳米粒子（nZnO）和壳聚糖（CS）/纳米晶羟基磷灰石（nHA），通过氧化自聚合、纳米粒子沉积、溶剂浇铸和蒸发方法，在 Ti 植入物上开发出了具有分级杂化结构的生物涂层，以提高其抗菌活性和成骨能力。多孔网状 Ti 基底上的 PDA 修饰极大地降低了表面粗糙度、润湿性、蛋白质吸附，并提供了与沉积的 nZnO 的高附着力。此外，在 PDA 涂层的 Ti 表面上掺入 nZnO 会影响表面结构和润湿性，显著抑制金黄色葡萄球菌和大肠杆菌的生长。此外，在 nZnO 修饰的 Ti 表面上掺杂 CS/nHA 涂层可显著提高细胞相容性，并通过上调碱性磷酸酶的蛋白表达来增强 MC3T3-E1 细胞的成骨分化。这项工作为开发具有长效生物活性的 Ti 植入物提供了一种有前途的替代方案，以实现强大的抗菌能力和增强的骨形成，用于潜在的牙科/骨科应用。
