Colleges of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
Colleges of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
Talanta. 2021 Aug 15;231:122331. doi: 10.1016/j.talanta.2021.122331. Epub 2021 Apr 9.
Cysteine (Cys) is a biological thiol. Aberrant changes in thiol levels are associated with the development and pathogenesis of various diseases, including liver damage, Alzheimer's disease, weakness, and cardiovascular diseases. Therefore, thiol detection in biological samples has great importance in health monitoring and disease prediction. In this study, we developed a ratiometric fluorescence nanosensor combined with carbon dots (CDs)-doped mesoporous silica and fluorescein-based fluorescent probes loaded in pores for Cys detection. The nanosensor emitted fluorescence at 450 nm upon excitation at 370 nm. In the presence of Cys, the fluorescence emission from the probe could be selectively enhanced, whereas that from CDs could be changed. Thus, a ratiometric fluorescent sensor was developed. This sensor can eliminate the potential influence of background fluorescence and other analyte-independent external environmental factors. The nanosensor was utilized to monitor Cys levels in human serum, and satisfactory results were obtained. Results indicated that the nanosensor can be utilized as an excellent fluorescent nanocomposite material in practical biological applications.
半胱氨酸（Cys）是一种生物硫醇。硫醇水平的异常变化与各种疾病的发展和发病机制有关，包括肝损伤、阿尔茨海默病、虚弱和心血管疾病。因此，生物样本中的硫醇检测在健康监测和疾病预测中具有重要意义。在本研究中，我们开发了一种比率荧光纳米传感器，该传感器结合了载有基于荧光素的荧光探针的碳点（CDs）掺杂介孔硅，用于 Cys 检测。纳米传感器在 370nm 激发时发出 450nm 的荧光。在 Cys 的存在下，探针的荧光发射可以被选择性增强，而 CDs 的荧光发射可以被改变。因此，开发了一种比率荧光传感器。该传感器可以消除背景荧光和其他与分析物无关的外部环境因素的潜在影响。该纳米传感器用于监测人血清中的 Cys 水平，获得了令人满意的结果。结果表明，该纳米传感器可用作实际生物应用中的优异荧光纳米复合材料。
