Miao Chen-Fang, Guo Xian-Zhong, Zhang Xin-Tian, Lin Yin-Ning, Han Wen-Di, Huang Zheng-Jun, Weng Shao-Huang
Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University Fuzhou 350122 China
Department of Pharmacy, First Affiliated Hospital of Fujian Medical University Fuzhou Fujian 350005 P. R. China
RSC Adv. 2021 Oct 25;11(55):34525-34532. doi: 10.1039/d1ra07078a.
The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as a donor are rarely reported as a drug monitor. In this work, CDs with an appropriate emitting wavelength at 480 nm and excitation around 370 nm were prepared by hydrothermal approach and HCl post-treatment. O-Phenylenediamine (OPD) can be oxidized by Cu to produce yellow fluorescent 2,3-diaminophenazine (oxOPD) in the system of Cu and OPD (Cu-OPD). Correspondingly, a remarkable FRET from CDs to oxOPD in the system of CDs, Cu and OPD (CDs-Cu-OPD) was fabricated with the quenching illustration of CDs, but emitting property of oxOPD. Attributed to the chelation ability of DEF on Cu, the inhibitory effects of DEF on the Cu-triggered oxidative capability reduced the FRET system by the decreased oxOPD. Thus, the recovered CDs at and decreased oxOPD at were found through a ratiometric mode by the addition of DEF in CDs-Cu-OPD for the DEF assay. The FRET behavior of CDs and oxOPD in CDs-Cu-OPD was proved clearly through the calculation of the association constant, binding constant, number of binding sites, and the distance between the donor and acceptor. Furthermore, this ratiometric method exhibited promising analytical performance for DEF with the application in real samples. The implementation of this work expands the application field of CDs and OPD oxidation in drug monitoring, and even other biological analyses through ratiometric strategy.
对于需要排铁的患者，去铁胺（DEF）监测具有重要的临床意义。然而，兼具实用性和简便性的分析方法有限。此外，基于碳点（CDs）作为供体的荧光共振能量转移（FRET）的比率荧光策略作为药物监测方法鲜有报道。在本研究中，通过水热法和盐酸后处理制备了发射波长为480 nm、激发波长约为370 nm的碳点。在铜和邻苯二胺（OPD）体系（Cu-OPD）中，OPD可被铜氧化生成黄色荧光物质2,3-二氨基吩嗪（oxOPD）。相应地，在碳点、铜和OPD体系（CDs-Cu-OPD）中构建了从碳点到oxOPD的显著FRET，表现为碳点猝灭而oxOPD发光。由于DEF对铜的螯合能力，DEF对铜触发的氧化能力的抑制作用通过减少oxOPD降低了FRET体系。因此，在CDs-Cu-OPD中加入DEF用于DEF检测时，通过比率模式发现了在[具体条件1]下碳点荧光恢复以及在[具体条件2]下oxOPD荧光降低。通过计算结合常数、结合位点数以及供体与受体之间的距离，明确证实了CDs-Cu-OPD中CDs与oxOPD之间的FRET行为。此外，该比率法在实际样品检测中对DEF表现出良好的分析性能。本研究工作的开展拓展了碳点和OPD氧化在药物监测以及甚至其他生物分析中的应用领域，通过比率策略实现了更广泛的应用。
