Yang Feng-Lian, Wei Yu-Xia, Liao Bi-Yun, Wei Gui-Jiang, Qin Hai-Mei, Pang Xiao-Xia, Wang Jun-Li
Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China.
Center of Reproductive and Genetic Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Guangxi, China.
Genomics. 2021 Jul;113(4):2276-2289. doi: 10.1016/j.ygeno.2021.03.036. Epub 2021 May 7.
BACKGROUND/AIM: There has been increasing evidence for the function of long non-coding RNA (lncRNA) in nasopharyngeal carcinoma (NPC). We aim to delve into the position of lncRNA HOX antisense intergenic RNA (HOTAIR), together with enhancer of zeste homolog 2 (EZH2), E-cadherin and trimethylation of lysine 27 on histone H3 (H3K27me3) in NPC.
HOTAIR, EZH2, and E-cadherin expression in NPC tissues and cells were tested. NPC cell biological functions were examined through gain-of and loss-of function assays. The mechanism of lncRNA HOTAIR/E-cadherin/EZH2/H3K27 axis in NPC was decoded.
LncRNA HOTAIR and EZH2 were highly expressed in NPC, and E-cadherin was lowly expressed. Down-regulation of HOTAIR or EZH2 inhibited NPC cell progression and tumor growth. HOTAIR recruited histone methylase EZH2 to mediate trimethylation of H3K27 and regulated E-cadherin expression.
HOTAIR inhibits E-cadherin by stimulating the trimethylation of H3K27 to promote NPC cell progression through recruiting histone methylase EZH2.
背景/目的：越来越多的证据表明长链非编码RNA（lncRNA）在鼻咽癌（NPC）中发挥作用。我们旨在深入研究lncRNA HOX反义基因间RNA（HOTAIR）以及zeste同源物2（EZH2）增强子、E-钙黏蛋白和组蛋白H3赖氨酸27三甲基化（H3K27me3）在鼻咽癌中的作用。
检测鼻咽癌组织和细胞中HOTAIR、EZH2和E-钙黏蛋白的表达。通过功能获得和功能丧失实验检测鼻咽癌细胞的生物学功能。解析lncRNA HOTAIR/E-钙黏蛋白/EZH2/H3K27轴在鼻咽癌中的作用机制。
lncRNA HOTAIR和EZH2在鼻咽癌中高表达，而E-钙黏蛋白低表达。下调HOTAIR或EZH2可抑制鼻咽癌细胞进展和肿瘤生长。HOTAIR招募组蛋白甲基化酶EZH2介导H3K27三甲基化并调节E-钙黏蛋白表达。
HOTAIR通过招募组蛋白甲基化酶EZH2刺激H3K27三甲基化来抑制E-钙黏蛋白，从而促进鼻咽癌细胞进展。
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