Adolpho Letícia Faustino, Gomes Maria Paula Oliveira, Freitas Gileade Pereira, Bighetti-Trevisan Rayana Longo, Ramos Jaqueline Isadora Reis, Campeoti Gabriela Hernandes, Zatta Guilherme Crepi, Almeida Adriana Luisa Gonçalves, Tarone Adriana Gadioli, Marostica-Junior Mario Roberto, Rosa Adalberto Luiz, Beloti Marcio Mateus
Bone Research Lab, Ribeirão Preto School of Dentistry, University of São Paulo, Av do Café s/n, Ribeirão Preto 14040-904, SP, Brazil.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Federal University of Goiás, Avenida Universitária, s/n-Setor Leste Universitário, Goiânia 74605-020, GO, Brazil.
Biology (Basel). 2024 Jul 16;13(7):526. doi: 10.3390/biology13070526.
Therapies to prevent osteoporosis are relevant since it is one of the most common non-communicable human diseases in the world and the most prevalent bone disorder in adults. Since jaboticaba peel extract (JPE) added to the culture medium enhanced the osteogenic potential of mesenchymal stem cells (MSCs) derived from osteoporotic rats, we hypothesized that JPE prevents the development of ovariectomy-induced osteoporosis. Ovariectomized rats were treated with either JPE (30 mg/kg of body weight) or its vehicle for 90 days, starting 7 days after the ovariectomy. Then, the femurs were subjected to microcomputed tomography and histological analyses, and the osteoblast and adipocyte differentiation of MSCs was evaluated. JPE attenuated ovariectomy-induced bone loss, as evidenced by higher bone volume/total volume and trabecular number, along with lower trabecular separation and bone marrow adiposity. These protective effects of JPE on bone tissue are due to its ability to prevent the imbalance between osteoblast and adipocyte differentiation of MSCs, since, compared with MSCs derived from ovariectomized rats treated with vehicle, MSCs treated with JPE exhibited higher gene and protein expression of osteogenic markers and extracellular matrix mineralization, as well as lower gene expression of adipogenic markers. These data highlight the potential therapeutic use of JPE to prevent osteoporosis.
预防骨质疏松症的疗法具有重要意义，因为它是世界上最常见的人类非传染性疾病之一，也是成年人中最普遍的骨骼疾病。由于添加到培养基中的巴西葡萄果皮提取物（JPE）增强了骨质疏松大鼠来源的间充质干细胞（MSC）的成骨潜能，我们推测JPE可预防卵巢切除诱导的骨质疏松症。卵巢切除大鼠在卵巢切除术后7天开始，用JPE（30mg/kg体重）或其赋形剂治疗90天。然后，对股骨进行微型计算机断层扫描和组织学分析，并评估MSC的成骨细胞和脂肪细胞分化。JPE减轻了卵巢切除诱导的骨质流失，较高的骨体积/总体积和小梁数量，以及较低的小梁间距和骨髓脂肪含量证明了这一点。JPE对骨组织的这些保护作用归因于其防止MSC成骨细胞和脂肪细胞分化失衡的能力，因为与用赋形剂处理的卵巢切除大鼠来源的MSC相比，用JPE处理的MSC表现出更高的成骨标志物基因和蛋白质表达以及细胞外基质矿化，以及更低的脂肪生成标志物基因表达。这些数据突出了JPE预防骨质疏松症的潜在治疗用途。
