Mukerjee S, Tekwani B L, Tripathi L M, Maitra S C, Visen P K, Katiyar J C, Ghatak S
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Exp Mol Pathol. 1988 Aug;49(1):50-61. doi: 10.1016/0014-4800(88)90020-2.
Ancylostoma ceylanicum infection in golden hamsters (Mesocricetus auratus) caused marked biochemical and histopathological derangements. Jejunum, the primary site of infection, showed pronounced alterations compared with liver. Though the biochemical composition of jejunum was not significantly altered, activities of a few lysosomal enzymes were enhanced during hookworm infection. Marked damage to mitochondrial and microsomal membranes was reflected in changes in the activities of the marker enzymes from jejunal tissue. Lipid content, especially phospholipids and neutral lipids of hepatic tissue, exhibited marked elevation. Levels of hexokinase, phosphofructokinase, and lactate dehydrogenase were enhanced in jejunal as well as hepatic tissues, indicating activation of the glycolytic machinery during hookworm infection. A decrease in the levels of mucosal disaccharidases indicated damage to intestinal brush border membranes. However, alkaline phosphatase activity was increased in intestinal mucosa during the infection. Light microscopic examination of jejunal tissue revealed peeling off of the upper epithelial layer, activation of the goblet cells, and thickening of muscularis mucosa. However, hepatic tissue did not show gross alterations, except for slight necrosis in the centrilobular region.
锡兰钩虫感染金黄仓鼠（Mesocricetus auratus）会导致明显的生化和组织病理学紊乱。空肠作为主要感染部位，与肝脏相比显示出明显的改变。虽然空肠的生化组成没有显著变化，但在钩虫感染期间，一些溶酶体酶的活性增强。空肠组织中标记酶活性的变化反映了线粒体和微粒体膜受到的明显损伤。肝组织的脂质含量，尤其是磷脂和中性脂质，显著升高。空肠和肝组织中己糖激酶、磷酸果糖激酶和乳酸脱氢酶的水平升高，表明在钩虫感染期间糖酵解机制被激活。黏膜二糖酶水平的降低表明肠刷状缘膜受到损伤。然而，感染期间肠黏膜中的碱性磷酸酶活性增加。空肠组织的光镜检查显示上皮上层脱落、杯状细胞活化和黏膜肌层增厚。然而，肝组织除了小叶中心区域有轻微坏死外，没有明显改变。
