Relationship between acrosome reaction and in vitro fertilization of zona-free hamster eggs by bonnet monkey (Macaca radiata) spermatozoa.
作者信息
Peter J, Menezes J, Jayaraman S
出版信息
Arch Androl. 1985;14(2-3):227-30. doi: 10.3109/01485018508988304.
PMID:3933444
Abstract
The relationship between acrosome reaction, as studied by FITC-RCA staining technique, and the penetration of bonnet monkey spermatozoa into zona-free hamster eggs was investigated. The acrosomes of unreacted spermatozoa fluoresced, whereas those of acrosome-reacted sperm did not fluoresce owing to decreased binding of the lectin. The percentage of acrosome-reacted sperm increased following 3 h of incubation in BWW medium. The assessment of acrosome reaction by the FITC-RCA staining technique correlates well with the in vitro fertilization of zona-free hamster eggs.
摘要
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