Kadir Eniola Risikat, Imam Aminu, Olajide Olayemi Joseph, Ajao Moyosore Saliu
Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.
Centre for Studies in Behavioural Neurobiology, Concordia University, Montreal, QC, Canada.
Anat Cell Biol. 2021 Jun 30;54(2):212-224. doi: 10.5115/acb.20.215.
Bisphenol A is a chemical used primarily as a monomer in the production of polycarbonate plastics and epoxy resins. It is a synthetic chemical compound that is produced in billions of pounds annually, and tagged as an endocrine disruptor. Bisphenol A is a high production synthetic chemical compound that is used in the production of many consumables and equipments of daily consumption and use by man. Growing interest in possible health threats posed by endocrine disrupting chemicals (bisphenol-A inclusive), as these substances are in our environment, food, and many consumer products. Therefore, this study aims to determine bisphenol-A effects on the hypothalamo-pituitary-ovarian axis, and role of melatonin in this regard. Forty-two Wistar rats were bred, grouped into 7, with each group consisting of 6 rats. Experimental groups were administered low and high doses of bisphenol-A and melatonin, starting from day 19, and was continued for 7 weeks orally. They were left to develop into full adults and were sacrificed on day 120±4 days. Blood samples, hypothalamus, pituitary and ovarian tissues were excised for biochemical and tissue antioxidants assays as well as genetic studies. Results show elevated gonadotropin and androgen levels. There was disruption of reactive oxygen species in the ovarian tissues, as well as alterations in the expression of genes that regulate reproduction at the hypothalamus and pituitary levels. Conclusion of early exposure to bisphenol-A is associated with prolonged duration of disruption of reproductive functions in female Wistar rats, which persist long after cessation of the exposure. Melatonin antioxidant effects give some promising outturns against bisphenol-A induced toxicities.
双酚A是一种主要用作生产聚碳酸酯塑料和环氧树脂单体的化学物质。它是一种合成化合物，年产量达数十亿磅，并被标记为内分泌干扰物。双酚A是一种高产量的合成化合物，用于生产许多人类日常消费和使用的消费品及设备。人们越来越关注内分泌干扰化学物质（包括双酚A）可能对健康造成的威胁，因为这些物质存在于我们的环境、食物和许多消费品中。因此，本研究旨在确定双酚A对下丘脑 - 垂体 - 卵巢轴的影响，以及褪黑素在这方面的作用。饲养了42只Wistar大鼠，分为7组，每组6只。从第19天开始，对实验组口服低剂量和高剂量的双酚A及褪黑素，并持续7周。让它们发育为成年大鼠，并在第120±4天处死。采集血液样本、下丘脑、垂体和卵巢组织，用于生化和组织抗氧化剂检测以及基因研究。结果显示促性腺激素和雄激素水平升高。卵巢组织中的活性氧物种受到破坏，并且下丘脑和垂体水平上调节生殖的基因表达也发生了改变。结论是，早期接触双酚A与雌性Wistar大鼠生殖功能紊乱的持续时间延长有关，这种紊乱在接触停止后仍会持续很长时间。褪黑素的抗氧化作用对双酚A诱导的毒性产生了一些有希望的结果。
