Abdollahifar Mohammad-Amin, Azad Nahid, Sajadi Ensieh, Shams Mofarahe Zahra, Zare Fatemeh, Moradi Ali, Rezaee Fatereh, Gholamin Mohammad, Abdi Shabnam
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran.
Anat Cell Biol. 2019 Jun;52(2):196-203. doi: 10.5115/acb.2019.52.2.196. Epub 2019 Jun 30.
Ovarian aging is related to the reduction of oocyte quality and ovarian follicles reservation leading to infertility. Vitamin C is a natural antioxidant which may counteract with adverse effects of aging in the ovary. The aim of this study was to evaluate the possible effect of vitamin C on NMRI mice ovarian aging according to the stereological study. In this experimental study, 36 adult female mice (25-30 g) were divided into two groups: control and vitamin C. Vitamin C (150 mg/kg/day) were administered by oral gavage for 33 weeks. Six animals of each group were sacrificed on week 8, 12, and 33, and right ovary samples were extracted for stereology analysis. Our data showed that the total volume of ovary, cortex, medulla and corpus luteum were significantly increased in vitamin C group in comparison to the control groups (≤0.05). In addition, the total number of primordial, primary, secondary, and antral follicles as well as granulosa cells were improved in vitamin C group in compared to the control groups (≤0.05). No significant difference was observed in total volume of oocytes in antral follicles between control and vitamin C groups. Our data showed that vitamin C could notably compensate undesirable effects of ovarian aging in a mouse model.
卵巢衰老与卵母细胞质量下降及卵巢卵泡储备减少有关，进而导致不孕。维生素C是一种天然抗氧化剂，可能抵消卵巢衰老的不良影响。本研究的目的是根据体视学研究评估维生素C对NMRI小鼠卵巢衰老的可能影响。在这项实验研究中，36只成年雌性小鼠（25 - 30克）被分为两组：对照组和维生素C组。维生素C（150毫克/千克/天）通过口服灌胃给药33周。每组6只动物在第8周、12周和33周时处死，提取右侧卵巢样本进行体视学分析。我们的数据显示，与对照组相比，维生素C组卵巢、皮质、髓质和黄体的总体积显著增加（≤0.05）。此外，与对照组相比，维生素C组原始卵泡、初级卵泡、次级卵泡和窦状卵泡以及颗粒细胞的总数有所改善（≤0.05）。对照组和维生素C组窦状卵泡中卵母细胞的总体积未观察到显著差异。我们的数据表明，维生素C可以显著补偿小鼠模型中卵巢衰老的不良影响。
