基于 RNAseq 的轻度低温对放射性肺损伤保护作用的转录组分析。
Transcriptome analysis of mild hypothermia protection against radiationinduced rat lung injury based on RNAseq.
机构信息
Drug Safety Evaluation Center of China Institute for Radiation Protection; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury; Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021 Apr 28;46(4):345-350. doi: 10.11817/j.issn.1672-7347.2021.190727.
OBJECTIVES
To analyze the differentially expressed genes (DEGs) with radiation-induced rat lung injury, and to reveal the protective mechanism for mild hypothermia in the radiation-induced lung injury in rats at the transcriptome level.
METHODS
A total of 10 male SD rats aged 6-8 weeks were randomly divided into 2 groups to establish a rat model of radiation-induced lung injury, and one group was treated with mild hypothermia. RNA was extracted from left lung tissue of each group, and sequenced by BGISEQ-500 platform. Significance analysis of DEGs was carried out by edgeR software. Gene ontology (GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to analyze the gene function. Then 5 key DEGs were verified by real-time reverse transcription PCR (real-time RT-PCR).
RESULTS
There were 2 790 DEGs (false discovery rate<0.001, |log(fold change)|>1) in the mild hypothermia group compared with the model group, in which 2 257 genes were up-regulated and 533 genes were down-regulated. When real-time RT-PCR was used to validate the 5 key genes, the result was consistent with the RNA-seq. GO functional enrichment analysis showed that these DEGs were related to cell binding, metabolic process and cell membrane structure, etc. KEGG pathway enrichment analysis showed that these genes were involved in important biological pathways such as cell adhesion molecules, mammalian target of rapamycin, tight junction, and NF-κB.
CONCLUSIONS
The DEGs and pathways related to mild hypothermia protection against radiation-induced lung injury in rats are obtained, which provides an experimental basis for the protection of mild hypothermia against radiation-induced lung injury.
目的
分析放射性肺损伤大鼠差异表达基因（DEGs），从转录组水平揭示亚低温对放射性肺损伤的保护作用机制。
方法
将 10 只 6～8 周龄雄性 SD 大鼠随机分为 2 组，建立放射性肺损伤大鼠模型，其中 1 组给予亚低温处理。提取各组左肺组织 RNA，采用 BGISEQ-500 平台进行测序。采用 edgeR 软件对 DEGs 进行显著性分析，采用基因本体（GO）功能富集分析和京都基因与基因组百科全书（KEGG）通路富集分析对基因功能进行分析，然后采用实时逆转录 PCR（real-time RT-PCR）验证 5 个关键 DEGs。
结果
亚低温组与模型组比较，有 2790 个 DEGs（假发现率<0.001，|log（fold change）|>1），其中 2257 个基因上调，533 个基因下调。用 real-time RT-PCR 验证 5 个关键基因，结果与 RNA-seq 一致。GO 功能富集分析显示，这些 DEGs 与细胞结合、代谢过程和细胞膜结构等有关。KEGG 通路富集分析显示，这些基因参与细胞黏附分子、哺乳动物雷帕霉素靶蛋白、紧密连接和 NF-κB 等重要的生物学途径。
结论
获得了与亚低温保护放射性肺损伤相关的 DEGs 和通路，为亚低温防治放射性肺损伤提供了实验依据。
Zotero 插件