Tong Shan, Wang Huan, A Li-Sha, Bai Ta-Na, Gong Ju-Hua, Jin Wen-Jie, Dai Li-Li, Ba Gen-Na, Cho Sung-Bo, Fu Ming-Hai
School of Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia Autonomous Region, China.
World J Gastroenterol. 2021 Apr 28;27(16):1770-1784. doi: 10.3748/wjg.v27.i16.1770.
Sulongga-4 (SL-4) is a herbal formula used in traditional Mongolian medical clinics for the treatment of peptic ulcers and gastroenteritis, even though its pharmacological mechanism has not been well characterized.
To evaluate the protective effect and identify the mechanisms of action of SL-4 on gastroduodenal ulcer induced by pyloric ligation (PL) in rats.
PL was performed to induce gastric and duodenal ulcers in rats, which were then treated with oral SL-4 (1.3, 2.6, or 3.9 g/kg per day) for 15 d. PL-induced gastroduodenal ulceration. Therapeutic effects were characterized by pathological and histological evaluations and inflammatory indicators were analyzed by enzyme-linked immunosorbent assay. Microarray analyses were conducted to identify gene expression profiles of gastroduodenal tissue in PL rats with or without SL-4 treatment. The candidate target genes were selected and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR).
SL-4 decreased histopathological features in the PL-induced ulcerated rats. SL-4 significantly ( < 0.05) decreased expression of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, endotoxin, platelet-activating factor, and increased prostaglandin E2 and epidermal growth factor in ulcer tissue. Microarray analysis was used to identify a panel of candidate target genes for SL-4 acting on PL-induced ulceration. Genes included some complement and coagulation cascade and retinol metabolism pathways that are closely associated with inflammatory responses and gastric mucosal protective mechanisms. qRT-PCR showed that altered expression of the selected genes, such as , , , and was consistent with the microarray results.
SL-4 exerts protective effects against PL-induced gastroduodenal ulcers reducing inflammatory cytokines and elevating expression of gastric acid inhibitory factors. Downregulation of and genes in retinol metabolism and upregulation of and genes in the complement and coagulation cascades pathways are possibly involved in SL-4-mediated protection against gastroduodenal ulcer.
苏龙嘎 - 4（SL - 4）是一种蒙医传统方剂，用于治疗消化性溃疡和肠胃炎，但其药理机制尚未完全明确。
评估SL - 4对大鼠幽门结扎（PL）诱导的胃十二指肠溃疡的保护作用，并确定其作用机制。
通过幽门结扎诱导大鼠胃和十二指肠溃疡，然后用SL - 4（每天1.3、2.6或3.9 g/kg）口服治疗15天。观察PL诱导的胃十二指肠溃疡情况。通过病理和组织学评估来表征治疗效果，并通过酶联免疫吸附测定分析炎症指标。进行基因芯片分析以鉴定经或未经SL - 4治疗的PL大鼠胃十二指肠组织的基因表达谱。通过定量逆转录聚合酶链反应（qRT - PCR）选择并验证候选靶基因。
SL - 4减轻了PL诱导的溃疡大鼠的组织病理学特征。SL - 4显著（<0.05）降低了溃疡组织中肿瘤坏死因子-α、白细胞介素（IL）-1β、IL - 6、内毒素、血小板活化因子的表达，并增加了前列腺素E2和表皮生长因子的表达。基因芯片分析用于鉴定SL - 4作用于PL诱导溃疡的一组候选靶基因。这些基因包括一些与炎症反应和胃黏膜保护机制密切相关的补体和凝血级联以及视黄醇代谢途径。qRT - PCR表明，所选基因如 、 、 、 的表达变化与基因芯片结果一致。
SL - 4对PL诱导的胃十二指肠溃疡具有保护作用，其机制可能是通过减少炎症细胞因子并提高胃酸抑制因子的表达。视黄醇代谢中 和 基因的下调以及补体和凝血级联途径中 和 基因的上调可能参与了SL - 4介导的胃十二指肠溃疡保护作用。
