College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, China.
CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety & CAS-HKU Joint Laboratory of Metallomics on Health and Environment, and Beijing Metallomics Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
Front Immunol. 2021 Apr 22;12:676773. doi: 10.3389/fimmu.2021.676773. eCollection 2021.
Perovskite nanomaterials (NMs) possess excellent physicochemical properties and have promising applications in light-emitting diodes (LEDs), lasers, photodetectors, and artificial synapse electronics. Potential exposure to these NMs happens in the manufacture and application of the perovskite-based products, however, the biological safety of these NMs is still unknown. Here, we used the LaNiO NM (LNO), a typical kind of perovskite nanostructures to study the interaction with macrophages (J774A.1) and to explore its biological effects at the cellular level. Firstly, we characterized the properties of LNO including the size, shape, and crystal structure using Transmission electronic microscope (TEM), Dynamic lighting scattering (DLS), and X-ray diffraction (XRD). Secondly, to gain a better understanding of the biological effect, we evaluated the effect of LNO on cell viability and found that LNO induced cell autophagy at a concentration of 5 μg/ml and influenced the inflammatory response based on RT-PCR result. Finally, we demonstrated the mechanism that LNO causes cell autophagy and immune response is probably due to the metal ions released from LNO in acidic lysosomes, which triggered ROS and increased lysosomal membrane permeation. This study indicates the safety aspect of perovskite NMs and may guide the rational design of perovskite NMs with more biocompatibility during their manufacture and application.
钙钛矿纳米材料（NMs）具有优异的物理化学性质，在发光二极管（LEDs）、激光、光电探测器和人工突触电子学等领域有广阔的应用前景。这些 NMs 可能在钙钛矿基产品的制造和应用中被潜在暴露，但这些 NMs 的生物安全性仍不清楚。在这里，我们使用 LaNiO NM（LNO），一种典型的钙钛矿纳米结构，来研究与巨噬细胞（J774A.1）的相互作用，并在细胞水平上探索其生物学效应。首先，我们用透射电子显微镜（TEM）、动态光散射（DLS）和 X 射线衍射（XRD）来表征 LNO 的性质，包括尺寸、形状和晶体结构。其次，为了更好地了解生物效应，我们评估了 LNO 对细胞活力的影响，发现 LNO 在 5μg/ml 的浓度下诱导细胞自噬，并根据 RT-PCR 结果影响炎症反应。最后，我们证明了 LNO 引起细胞自噬和免疫反应的机制可能是由于 LNO 在酸性溶酶体中释放出金属离子，触发 ROS 并增加溶酶体膜通透性。本研究表明了钙钛矿 NMs 的安全性，并可能为在制造和应用过程中设计具有更高生物相容性的钙钛矿 NMs 提供指导。
