Shen Jiayu, Zhang Hongwei, Lu Chen, Gu Jun, Zhang Yu, Hu Jia
Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Exp Ther Med. 2021 Jun;21(6):635. doi: 10.3892/etm.2021.10067. Epub 2021 Apr 15.
Long non-coding RNAs (lncRNAs) have been reported to be involved in various biological processes, including cell proliferation and apoptosis. However, the expression profiles of lncRNAs in patients with vein graft restenosis remain unknown. In the present study, the time-dependent expression profiles of genes in vein bypass grafting models were examined by microarray analysis. A total of 2,572 lncRNAs and 1,652 mRNAs were identified to be persistently significantly differentially expressed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed to investigate the functions of these lncRNAs. A total of 360 lncRNAs and 135 protein-coding genes were predicted to be involved in the vascular remodeling process. Co-expression network analysis revealed the association between 194 lncRNAs and seven associated protein-coding genes, including transforming growth factor-β1, Fes, Yes1 associated transcriptional regulator, sphingosine-1-phosphate receptor 1, Src, insulin receptor and melanoma cell adhesion molecule. Moreover, reverse transcription-quantitative PCR results supported those of the microarray data, and overexpression of AF062402, which regulates the transcription of Src, stimulated the proliferation of primary vascular smooth muscle cells. The findings of the present study may facilitate the development of novel therapeutic targets for vein graft restenosis and may help to improve the prognosis of patients following coronary artery bypass grafting.
据报道，长链非编码RNA（lncRNAs）参与多种生物学过程，包括细胞增殖和凋亡。然而，lncRNAs在静脉移植物再狭窄患者中的表达谱仍不清楚。在本研究中，通过微阵列分析检测了静脉旁路移植模型中基因的时间依赖性表达谱。共鉴定出2572个lncRNAs和1652个mRNA持续存在显著差异表达。进行基因本体论和京都基因与基因组百科全书富集分析以研究这些lncRNAs的功能。预计共有360个lncRNAs和135个蛋白质编码基因参与血管重塑过程。共表达网络分析揭示了194个lncRNAs与7个相关蛋白质编码基因之间的关联，包括转化生长因子-β1、Fes、Yes1相关转录调节因子、鞘氨醇-1-磷酸受体1、Src、胰岛素受体和黑色素瘤细胞粘附分子。此外，逆转录定量PCR结果支持微阵列数据的结果，并且调节Src转录的AF062402的过表达刺激了原代血管平滑肌细胞的增殖。本研究结果可能有助于开发针对静脉移植物再狭窄的新型治疗靶点，并可能有助于改善冠状动脉旁路移植术后患者的预后。
