Dai Ying, Dou Zhihua, Zhou Rongrong, Luo Lin, Bian Li, Chen Yufeng, Tao Jinhua, Chen Zhixian
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
Nantong Third People's Hospital, Nantong University, Nantong 226006, Jiangsu, China.
J Anal Methods Chem. 2021 Apr 21;2021:5546446. doi: 10.1155/2021/5546446. eCollection 2021.
In this study, a new method was developed for the comprehensive quality evaluation (QE) of Thunb. (, named Yinchenhao in Chinese), which is one of the most commonly used herbal medicines (HMs). First, fingerprints of 31 batch samples of were determined by HPLC, the reference fingerprint was established, and the common peaks were assigned. Second, the components of common peaks in the HPLC fingerprints were identified by ultrafast liquid chromatography- (UFLC-) Q-TOF-MS/MS. Finally, the contents of the components unambiguously confirmed by reference substances were determined, and the correlation between the contents of chlorogenic acid and the contents of others was analyzed. The results showed that there were 20 common peaks in the HPLC fingerprints of 31 batch samples. The components of these 20 common peaks were identified as ten organic acids, eight flavonoids, and two others. Among nine organic acids such as 1-caffeoylquinic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid, three flavonoids such as rutin, hyperoside, and isoquercetin, and one other -hydroxyacetophenone, a total of 13 ones were unambiguously identified by comparison with reference substances; one caffeoylquinic acid glucoside and one flavone di-C-glucoside were detected in for the first time. There were some differences in the contents of 13 components in different samples; chlorogenic acid could be regarded as the quality marker of . The current established method in this study can be used for the comprehensive QE of and can also provide reference for the QE of the other HMs.
本研究建立了一种用于对最常用草药之一茵陈蒿（学名：Artemisia capillaris Thunb.，中药名为茵陈蒿）进行综合质量评价（QE）的新方法。首先，采用高效液相色谱法（HPLC）测定了31批茵陈蒿样品的指纹图谱，建立了对照指纹图谱并对共有峰进行了归属。其次，通过超快速液相色谱-（UFLC-）Q-TOF-MS/MS对HPLC指纹图谱中共有峰的成分进行了鉴定。最后，测定了经对照品明确确认的成分的含量，并分析了绿原酸含量与其他成分含量之间的相关性。结果表明，31批样品的HPLC指纹图谱中有20个共有峰。这20个共有峰的成分被鉴定为10种有机酸、8种黄酮类化合物和2种其他成分。在1-咖啡酰奎尼酸、新绿原酸、绿原酸、咖啡酸、隐绿原酸、1,3-二咖啡酰奎尼酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸和4,5-二咖啡酰奎尼酸等9种有机酸、芦丁、金丝桃苷和异槲皮苷等3种黄酮类化合物以及1种其他成分对羟基苯乙酮中，通过与对照品比较，共明确鉴定出13种；首次在茵陈蒿中检测到1种咖啡酰奎尼酸葡萄糖苷和1种黄酮二-C-葡萄糖苷。不同样品中13种成分的含量存在一定差异；绿原酸可被视为茵陈蒿的质量标志物。本研究建立的现行方法可用于茵陈蒿的综合质量评价，也可为其他草药的质量评价提供参考。
