Department of Parasite Disease Control and Prevention, Henan Provincial Center for Disease Control and Prevention, Henan Key Laboratory of Infectious Disease Microbiology, Zhengzhou, China.
Front Cell Infect Microbiol. 2021 Apr 23;11:644576. doi: 10.3389/fcimb.2021.644576. eCollection 2021.
Malaria remains a major public health issue in Nigeria, and Nigeria is one of the main sources of imported malaria in China. Antimalarial drug resistance is a significant obstacle to the control and prevention of malaria globally. The molecular markers associated with antimalarial drug resistance can provide early warnings about the emergence of resistance. The prevalence of antimalarial drug resistant genes and mutants, including , , , , and , was evaluated among the imported isolates from Nigeria in Henan, China, from 2012 to 2019. Among the 167 imported isolates, the wild-type frequency of , , , , and was 98.7, 63.9, 34.8, 3.1, and 3.1%, respectively. The mutation of was rare, with just two nonsynonymous (S693F and Q613H) and two synonymous mutations (C469C and G496G) identified from four isolates. The prevalence of mutation at codon 74-76 decreased year-by-year, while the prevalence of 86Y also decreased significantly with time. The prevalence of and mutants was high. Combined mutations of and had a high prevalence of the quadruple mutant IRN-G (39.0%), followed by the octal mutant IRN-VAGGS (17.0%). These molecular findings update the known data on antimalarial drug-resistance genes and provide supplemental information for Nigeria.
疟疾仍然是尼日利亚的一个主要公共卫生问题，尼日利亚也是中国输入性疟疾的主要来源地之一。抗疟药物耐药性是全球疟疾控制和预防的重大障碍。与抗疟药物耐药性相关的分子标记物可以提供耐药性出现的早期预警。2012 年至 2019 年，在中国河南从尼日利亚输入的 167 株 中，评估了抗疟药物耐药基因和突变体，包括 、 、 、 、 。在 167 株输入性 中，野生型频率分别为 、 、 、 和 为 98.7%、63.9%、34.8%、3.1%和 3.1%。 突变罕见，仅从 4 株分离株中鉴定出 2 个非同义（S693F 和 Q613H）和 2 个同义突变（C469C 和 G496G）。74-76 密码子 突变的流行率逐年下降，而 86Y 突变的流行率也随时间显著下降。 和 突变体的流行率较高。 和 联合突变的四重突变 IRN-G（39.0%）的流行率较高，其次是八重突变 IRN-VAGGS（17.0%）。这些分子发现更新了已知的抗疟药物耐药基因数据，并为尼日利亚提供了补充信息。
