Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan 523850, China.
Anal Methods. 2021 May 27;13(20):2283-2289. doi: 10.1039/d1ay00548k.
Chrysanthemum indici flos (CIF), a traditional functional beverage, has been used in China for thousands of years. However, research on the antioxidant properties and compounds in CIF is insufficient. In the current study, an online gradient extraction (OGE) coupled with a high performance liquid chromatography-ferric reducing antioxidant power (HPLC-FRAP) system for rapid identification of antioxidants in CIF was proposed. The CIF sample (0.3 mg) was extracted online by a gradient mobile phase (acetonitrile and 0.1% formic acid), separated on a Poroshell 120 SB-Aq column, and then detected for the anti-oxidative activity with the online FRAP assay. 36 peaks were found and 28 compounds were identified. Among them, 18 peaks showed antioxidant activity, six of which were detected in CIF for the first time. The total analytical time was less than 45 min, including sample extraction, separation, and antioxidant identification. The results indicate that the OGE-HPLC-FRAP system is a simple, efficient and rapid tool for screening antioxidants in CIF, which provides the scientific data of CIF's antioxidant research and is helpful to improve the quality evaluation of CIF.
菊花（CIF）是一种传统的功能性饮料，在中国已有数千年的历史。然而，关于 CIF 的抗氧化特性和化合物的研究还不够充分。在当前的研究中，提出了一种在线梯度萃取（OGE）与高效液相色谱-铁还原抗氧化能力（HPLC-FRAP）系统相结合的方法，用于快速鉴定 CIF 中的抗氧化剂。CIF 样品（0.3mg）通过梯度流动相（乙腈和 0.1%甲酸）在线提取，在 Poroshell 120 SB-Aq 柱上分离，然后通过在线 FRAP 测定法检测抗氧化活性。发现了 36 个峰，鉴定出 28 种化合物。其中，有 18 个峰显示出抗氧化活性，其中 6 种是首次在 CIF 中检测到的。总的分析时间不到 45 分钟，包括样品提取、分离和抗氧化鉴定。结果表明，OGE-HPLC-FRAP 系统是一种简单、高效、快速的筛选 CIF 中抗氧化剂的工具，为 CIF 的抗氧化研究提供了科学数据，有助于提高 CIF 的质量评价。
