Shen Qian-Hui, Huang Qi, Xie Ju-Ying, Wang Kun, Qian Zheng-Ming, Li De-Qiang
Guangdong Institute for Drug Control, NMPA Key Laboratory for Rapid Testing Technology of Drugs Guangzhou 510663 Guangdong Province China.
Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd No. 368, Zhen'an Middle Road, Chang'an Town Dongguan 523850 Guangdong Province China
RSC Adv. 2021 Jul 27;11(41):25646-25652. doi: 10.1039/d1ra04300e. eCollection 2021 Jul 19.
In the present study, a simple and efficient approach based on the online extraction-high performance liquid chromatography coupled with ABTS antioxidant assay (OLE-HPLC-ABTS) was established to quickly and directly analyze the antioxidants in . Through this system, the HPLC mobile phase a guard column packed with a sample was used for online extraction (OLE). The separation was performed on an Agilent Poroshell EC-C18 column with a gradient elution using 0.1% formic acid (A) and 0.1% formic acid-acetonitrile (B) as mobile phase systems and detected at a wavelength of 254 nm. Then, the separated compounds were reacted with the antioxidant solution (ABTS), and the response was recorded at a wavelength of 400 nm. The developed analytical method was successfully applied to samples, and eight antioxidants were identified. The established system integrated the online extraction, separation and online antioxidant detection, which is rapid, efficient, and suitable for the rapid screening of antioxidant compounds from solid sample mixtures.
在本研究中，建立了一种基于在线萃取-高效液相色谱联用ABTS抗氧化测定法（OLE-HPLC-ABTS）的简单高效方法，以快速直接分析[具体物质]中的抗氧化剂。通过该系统，使用装有[具体物质]样品的保护柱对HPLC流动相进行在线萃取（OLE）。在安捷伦Poroshell EC-C18柱上进行分离，以0.1%甲酸（A）和0.1%甲酸-乙腈（B）作为流动相系统进行梯度洗脱，并在254 nm波长处检测。然后，将分离出的化合物与抗氧化剂溶液（ABTS）反应，并在400 nm波长处记录响应。所开发的分析方法成功应用于[具体样品]，并鉴定出8种抗氧化剂。所建立的系统集成了在线萃取、分离和在线抗氧化剂检测，具有快速、高效的特点，适用于从固体样品混合物中快速筛选抗氧化化合物。
