Forensic Medicine & Toxicology Department, King George's Medical University, Lucknow, Uttar Pradesh, 226003, India.
Biochemistry Department, KGMU, Lucknow, India.
Mol Biol Rep. 2021 Apr;48(4):3245-3252. doi: 10.1007/s11033-021-06386-x. Epub 2021 May 10.
Alzheimer's disease is a common neurodegenerative disease in the elderly population and a leading cause of dementia. Genetics and environmental risk factors were considered to play a major role in the onset of the disease. This study aimed to examine the correlation between different metals levels and the gene expression in Alzheimer's patients with age-matched control subjects. Non- essential metals were measured in the whole blood due to its higher concentration in red blood corpuscles (RBCs) and essential biometals in the serum samples of Alzheimer's disease (AD) by using Inductively coupled plasma optical emission spectroscopy (ICP-OES) that allows the analysis and detection of the different elements at low levels. Gene expression level was performed by quantitative real-time PCR (qRT-PCR). In this study, the levels of Lead and Arsenic metals were not detected in the AD patient samples. Cadmium, Mercury, and Aluminum were found higher in cases as compared to controls with 0.009240 ± 0.0007707 (P = < 0.0001), 0.02332 ± 0.001041 (P = < 0.0001), and 0.09222 ± 0.02804 (P = 0.0087) respectively. Essential biometal like copper was higher 0.1274 ± 0.02453 (P = 0.0254) in cases, while iron 0.1117 ± 0.009599 (P = 0.0304) and zinc 0.03800 ± 0.003462 mg/L were found significantly lower as compared to controls. All targeted genes such as APP, PSEN1, PSEN2, and APOE4 were found up-regulated in AD patients. We concluded that there was no significant correlation between metals dyshomeostasis and gene expressions in this study.
阿尔茨海默病是老年人中常见的神经退行性疾病，也是痴呆症的主要原因。遗传和环境风险因素被认为在疾病的发生中起主要作用。本研究旨在检查不同金属水平与年龄匹配的对照组阿尔茨海默病患者基因表达之间的相关性。由于全血中红细胞（RBC）中浓度较高，因此用电感耦合等离子体发射光谱法（ICP-OES）测量非必需金属，而血清样本中的必需生物金属通过电感耦合等离子体发射光谱法（ICP-OES）测量。可以分析和检测低水平的不同元素。通过定量实时 PCR（qRT-PCR）进行基因表达水平的测定。在本研究中，在 AD 患者样本中未检测到铅和砷金属的水平。与对照组相比，病例中发现镉、汞和铝的水平更高，分别为 0.009240±0.0007707（P<0.0001）、0.02332±0.001041（P<0.0001）和 0.09222±0.02804（P=0.0087）。像铜这样的必需生物金属在病例中更高，为 0.1274±0.02453（P=0.0254），而铁 0.1117±0.009599（P=0.0304）和锌 0.03800±0.003462 mg/L 则明显低于对照组。所有靶向基因，如 APP、PSEN1、PSEN2 和 APOE4，在 AD 患者中均发现上调。我们得出的结论是，在这项研究中，金属动态平衡与基因表达之间没有显著相关性。
