Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
Anal Chem. 2021 May 25;93(20):7508-7515. doi: 10.1021/acs.analchem.1c00814. Epub 2021 May 10.
A smart electrochemiluminescent (ECL) sensor has been designed in this work. The sensing system consisted of Ag NPs-TiAlC nanosheets (Ag-TACS) as the self-luminous Faraday cage and biomimetic magnetic vesicles as the functional substrate. By engineering the structure and properties of TiAlC nanosheets to induce the Faraday-cage effect, the outer Helmholtz plane (OHP) was extended to contribute to ECL enhancement. Compared with the Faraday cage that further incorporated luminous materials, the self-luminous Faraday cage in the "direct label" model kept all the luminous materials on the OHP. Meanwhile, biomimetic magneticvesicles with highly efficient fluidity were used to improve the sensing efficiency and obtain a perfect Faraday-cage structure to enhance the ECL signals. The highest ECL enhancement (ca. 25 times) has been achieved by the synergistic effect of the Faraday cage and biomimetic magnetic vesicles. This sensing system was used to detect the wild-type K-ras gene in the colorectal tumor tissue. It provides not only an important guide for the novel ECL sensing concept but also a smart modulation system of the electromagnetic field.
本工作设计了一种智能电致化学发光（ECL）传感器。传感系统由 Ag NPs-TiAlC 纳米片（Ag-TACS）作为自发光法拉第笼和仿生磁性囊泡作为功能基底组成。通过对 TiAlC 纳米片的结构和性质进行工程设计，诱导法拉第笼效应，扩展外亥姆霍兹平面（OHP），有助于 ECL 增强。与进一步结合发光材料的法拉第笼相比，“直接标记”模型中的自发光法拉第笼将所有发光材料保留在 OHP 上。同时，利用具有高效流动性的仿生磁性囊泡来提高传感效率，并获得完美的法拉第笼结构，以增强 ECL 信号。法拉第笼和仿生磁性囊泡的协同作用实现了最高的 ECL 增强（约 25 倍）。该传感系统用于检测结直肠肿瘤组织中的野生型 K-ras 基因。它不仅为新型 ECL 传感概念提供了重要指导，而且为电磁场的智能调制系统提供了重要指导。
