Guo Yuchen, Nie Yixin, Wang Peilin, Li Zhenrun, Ma Qiang
Department of Gastrocolorectal Surgery, General Surgery Center, First Hospital of Jilin University, Changchun, 130021, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Talanta. 2023 Jul 1;259:124559. doi: 10.1016/j.talanta.2023.124559. Epub 2023 Apr 14.
Exosomes play an important role in the proliferation, adhesion and migration of cancer cells. In this study, we have developed a novel electrochemiluminescence (ECL) sensor based on MoS QDs-MXene heterostructure and Au NPs@biomimetic lipid layer to detect exosomal miRNA. MoS QDs-MXene heterostructure had been prepared as the luminescence probe. TiCT MXene nanosheets possessed the large specific surface area, excellent flexibility and superior conductivity. MoS QDs on the MXene nanosheets worked as the radiation center to generate strong ECL signal. Meanwhile, Au NPs with biomimetic lipid layer have been modified on the electrode, which retained the lipid dynamics and excellent antifouling property. When miRNA-135b was recognized on the Au NPs@biomimetic lipid layer, MoS QDs-MXene heterostructure was linked on the electrode and further extended the outer Helmholtz plane. As a result, the self-luminous Faraday cage-mode sensing system has been used to detect miRNA-135b from 30 fM to 20 nM with a detection limit of 10 fM. Furthermore, gastric cancer exosomal miRNA in the ascites of clinical patients has been detected successfully. The sensing system can be served as a versatile platform with huge application potential in the field of exosome detection.
外泌体在癌细胞的增殖、黏附和迁移中发挥着重要作用。在本研究中，我们基于MoS量子点- MXene异质结构和金纳米粒子@仿生脂质层开发了一种新型电化学发光（ECL）传感器，用于检测外泌体微小RNA。已制备MoS量子点- MXene异质结构作为发光探针。TiCT MXene纳米片具有大比表面积、优异的柔韧性和卓越的导电性。MXene纳米片上的MoS量子点作为辐射中心产生强烈的ECL信号。同时，在电极上修饰了具有仿生脂质层的金纳米粒子，其保留了脂质动力学和优异的抗污性能。当miRNA - 135b在金纳米粒子@仿生脂质层上被识别时，MoS量子点- MXene异质结构连接到电极上，并进一步扩展了外亥姆霍兹平面。结果，利用自发光法拉第笼模式传感系统检测miRNA - 135b的浓度范围为30 fM至20 nM，检测限为10 fM。此外，还成功检测了临床患者腹水中的胃癌外泌体微小RNA。该传感系统可作为一个具有巨大应用潜力的通用平台，用于外泌体检测领域。
