Structural Biology of Chromosome Segregation Laboratory, The Francis Crick Institute, London, United Kingdom.
Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute, London, United Kingdom.
PLoS One. 2021 May 10;16(5):e0251261. doi: 10.1371/journal.pone.0251261. eCollection 2021.
Chl1 is a member of the XPD family of 5'-3' DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1 has been shown to be required for chromosome segregation in yeast due to its role in the formation of persistent chromosome cohesion during S-phase. Here we present structural and biochemical data to show that Chl1 has the same overall domain organisation as other members of the XPD family, but with some conformational alterations. We also present data suggesting the insert domain in Chl1 regulates its DNA binding.
Chl1 是 XPD 家族的一员，属于 5'-3' DNA 解旋酶，在基因组维护和传递中发挥多种作用。它们具有多种独特的结构特征，包括在解旋酶结构域 1 中存在高度可变的部分有序插入。由于 Chl1 在 S 期形成持续的染色体凝聚物中的作用，它已被证明在酵母的染色体分离中是必需的。本文呈现的结构和生化数据表明，Chl1 具有与 XPD 家族其他成员相同的总体结构组织，但存在一些构象改变。我们还提供了数据表明 Chl1 中的插入结构域调节其 DNA 结合。
