NHC Key Laboratory of Food Safety Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing, 100021, China.
NHC Key Laboratory of Food Safety Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing, 100021, China.
Food Chem Toxicol. 2021 Jul;153:112254. doi: 10.1016/j.fct.2021.112254. Epub 2021 May 8.
11TPY001 is a transgenic maize that expresses the Aspergillus niger phyA2 gene which could significantly improve phosphorus bioavailability in monogastric animals. The present study was conducted to investigate the potential health effects of phytase transgenic maize 11TPY001 through a 90-day subchronic rodent feeding study. Maize grains from 11TPY001 or its parental counterpart maize OSL963 were incorporated into rodent diets at 12.5%, 25% and 50% concentrations by mass and administered to Sprague-Dawley rats (n = 10/sex/group) for 90 days. An additional control group of rats (n = 10/sex/group) were fed with common maize Zhengdan958 diets at 50% by mass. All formulated diets were nutritionally balanced. Body weights, food intake, hematology, serum chemistry, absolute and relative organ weights were measured, and gross as well as microscopic pathology were examined. Compared with rats fed OSL963 maize and the common maize diet groups, no adverse diet-related differences were observed in rats fed 11TPY001 maize diets with respect to clinical signs of toxicity, body weight/gain, food consumption/efficiency, hematology, clinical chemistry, organ weights, and gross and microscopic pathology. Under the conditions of this study, the results indicated that 11TPY001 did not cause any treatment related adverse effects in rats compared with its non-transgenic parental maize OSL963.
11TPY001 是一种转基玉米，表达了黑曲霉 phyA2 基因，可显著提高单胃动物的磷生物利用率。本研究通过 90 天的亚慢性啮齿动物喂养研究，旨在研究植酸酶转基因玉米 11TPY001 的潜在健康影响。11TPY001 或其亲本对照玉米 OSL963 的玉米粒以质量计分别以 12.5%、25%和 50%的浓度掺入啮齿动物饲料中，并以 50%的质量比喂给 Sprague-Dawley 大鼠（n = 10/性别/组）90 天。另外一组对照组大鼠（n = 10/性别/组）以 50%的质量比喂食普通玉米郑丹 958 饲料。所有配方饲料均具有均衡的营养。测量了体重、食物摄入量、血液学、血清化学、绝对和相对器官重量，并进行了大体和显微镜病理学检查。与喂食 OSL963 玉米和普通玉米的大鼠相比，喂食 11TPY001 玉米的大鼠在毒性的临床症状、体重/增重、食物消耗/效率、血液学、临床化学、器官重量以及大体和显微镜病理学方面没有观察到与饮食相关的不良差异。在本研究条件下，结果表明，与非转基因亲本玉米 OSL963 相比，11TPY001 未引起大鼠任何与治疗相关的不良影响。
