Department of Dermatology and Allergy, Herlev and Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
Coronel Institute of Occupational Health, Amsterdam UMC, Amsterdam Public Health Research Institute, University of Amsterdam, Amsterdam, The Netherlands.
Dermatology. 2021;237(6):940-945. doi: 10.1159/000514308. Epub 2021 May 10.
Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies.
需要用于特应性皮炎（AD）疾病严重程度和治疗反应的皮肤生物标志物。活检会导致疤痕，而胶带剥离代表了一种替代的、微创的角质层采样方法。在这项研究中，我们检测了 AD 患者皮肤活检和胶带条中细胞因子的基因表达。我们从健康对照组（n=6）和 AD 患者（n=12）中采集了皮肤活检和胶带条，在基线和外用莫米松糠酸酯 0.1%乳膏治疗 2 周后进行了采集。我们发现，与健康对照组皮肤相比，病变 AD 皮肤中的 IFN-γ、IL-13 和 IL-10 mRNA（活检）和 IL-1β 蛋白表达水平（胶带条）显著增加。外用皮质类固醇治疗导致 IL-13 和 IL-4R 的 mRNA 水平显著下降，但在胶带条中测量的细胞因子蛋白水平没有显著差异。最后，我们发现胶带条中的细胞因子水平与皮肤活检中的 mRNA 水平之间没有显著相关性。
