Cochrane A H, Matsumoto Y, Kamboj K K, Maracic M, Nussenzweig R S, Aikawa M
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10016.
Infect Immun. 1988 Aug;56(8):2080-8. doi: 10.1128/iai.56.8.2080-2088.1988.
The localization of Plasmodium brasilianum-derived antigens in short and long clefts within the cytoplasm of infected erythrocytes and in association with knobs of the host cell membrane was demonstrated by immunoelectron microscopy with monoclonal antibodies. Our results document that malaria-induced short and long clefts, previously distinguishable only by morphology, differ also in antigenic composition. Another parasite-derived antigen was found to be associated with the parasitophorous vacuole space in schizonts. In segmenters, this antigen was present in large amounts between merozoites and in the cytoplasm of infected cells. These antigens were characterized by biosynthetic labeling and gel electrophoresis.
利用单克隆抗体通过免疫电子显微镜证实，巴西疟原虫衍生抗原定位于受感染红细胞胞质内的短裂和长裂中，并与宿主细胞膜的隆起相关联。我们的结果表明，疟疾诱导的短裂和长裂以前仅通过形态学区分，其抗原组成也不同。另一种寄生虫衍生抗原被发现与裂殖体中的寄生泡腔相关。在裂殖子中，这种抗原大量存在于裂殖子之间以及受感染细胞的胞质中。这些抗原通过生物合成标记和凝胶电泳进行了表征。
