10-羟基-2E-癸烯酸（10HDA）不会促进无刺蜜蜂 Melipona scutellaris 的分蜂。
10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees.
机构信息
Institute of Biotechnology, Federal University of Uberlândia, Acre Street, 2E building, Uberlândia, MG, 38405-319, Brazil.
Department of Genetics, Harvard Medical School, Boston, MA, USA.
出版信息
Sci Rep. 2021 May 10;11(1):9882. doi: 10.1038/s41598-021-89212-5.
In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers-previous reported-whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA-the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation-was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.
在熊蜂属的蜜蜂中，差异喂养不足以完全解释雌性多态性。在这些蜜蜂中，有一种假设认为，除了环境成分（食物）外，遗传成分也参与了职别分化。这种机制尚未得到充分阐明，可能涉及表观遗传和代谢调节。在这里，我们验证了编码组蛋白去乙酰化酶 HDAC1 和 HDAC4 以及组蛋白乙酰转移酶 KAT2A 的基因在 Melipona scutellaris 的所有发育阶段都有表达，并且在发育阶段和职别之间存在波动。在幼虫中，HDAC 基因表现出与先前报道的 Juvenile Hormone 滴度相同的模式，而 HAT 基因则表现出相反的模式。我们还研究了幼虫和幼虫食物代谢组，但没有鉴定出可能诱导蜂王命运的化合物，橙花醇和 10-羟基-2E-癸烯酸（10HDA）。最后，我们证明了组蛋白去乙酰化酶抑制剂 10HDA——蜂王浆的主要脂质成分，因此是调控蜜蜂职别分化的潜在调节剂——不能促进 Melipona scutellaris 中蜂王的分化。我们的结果表明，表观遗传和激素调节可能协同作用，推动 Melipona 的职别分化，并且 10HDA 不是 Melipona scutellaris 职别分化的因素。
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