在超微结构水平上用氢氧化钠区分心脏弹性蛋白、胶原蛋白和微原纤维。
Differentiating cardiac elastin, collagen and microfibrils with NaOH at the ultrastructural level.
作者信息
Dickson C P, Robinson T F
机构信息
Albert Einstein College of Medicine, Division of Cardiology, Bronx, New York 10461.
出版信息
Histochemistry. 1988;89(2):105-7. doi: 10.1007/BF00489912.
PMID:3397303
Abstract
NaOH solutions extract elastin and collagen from epoxy-embedded thin sections containing rat cardiac connective tissue. Extraction results in a reverse staining effect of elastin and collagen ultrastructure. Microfibril contrast is enhanced by NaOH treatment. This phenomenon finds application in the possibility of differentiating elastin, collagen, and microfibrils at the ultrastructural level.
摘要
氢氧化钠溶液可从含有大鼠心脏结缔组织的环氧树脂包埋薄片中提取弹性蛋白和胶原蛋白。提取会导致弹性蛋白和胶原蛋白超微结构产生反向染色效果。通过氢氧化钠处理可增强微原纤维的对比度。这种现象在超微结构水平上区分弹性蛋白、胶原蛋白和微原纤维的可能性方面有应用价值。
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