• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状 RNA_30032 通过 miRNA-96-5p/HBEGF/KRAS 轴促进 UUO 模型小鼠的肾纤维化。

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis.

机构信息

Department of Emergency Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Emergency Medicine and Difficult Diseases Institute, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

出版信息

Aging (Albany NY). 2021 May 11;13(9):12780-12799. doi: 10.18632/aging.202947.

DOI:10.18632/aging.202947
PMID:33973871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8148471/
Abstract

In this study, we investigated the role of circular RNA_30032 (circRNA_30032) in renal fibrosis and the underlying mechanisms. The study was carried out using TGF-β1-induced BUMPT cells and unilateral ureteral obstruction (UUO)-induced mice, respectively, as and models. CircRNA_30032 expression was significantly increased by 9.15- and 16.6-fold on days 3 and 7, respectively, in the renal tissues of UUO model mice. In TGF-β1-treated BUMPT cells, circRNA_30032 expression was induced by activation of the p38 mitogen-activated protein kinase signaling pathway. Quantitative real-time PCR, western blotting and dual luciferase reporter assays showed that circRNA_30032 mediated TGF-β1-induced and UUO-induced renal fibrosis by sponging miR-96-5p and increasing the expression of profibrotic proteins, including HBEGF, KRAS, collagen I, collagen III and fibronectin. CircRNA_30032 silencing significantly reduced renal fibrosis in UUO model mice by increasing miR-96-5p levels and decreasing levels of HBEGF and KRAS. These results demonstrate that circRNA_30032 promotes renal fibrosis via the miR-96-5p/HBEGF/KRAS axis and suggest that circRNA_30032 is a potential therapeutic target for treatment of renal fibrosis.

摘要

在这项研究中，我们研究了环状 RNA_30032（circRNA_30032）在肾纤维化中的作用及其潜在机制。该研究分别使用 TGF-β1 诱导的 BUMPT 细胞和单侧输尿管梗阻（UUO）诱导的小鼠作为 和 模型进行。在 UUO 模型小鼠的肾脏组织中，circRNA_30032 的表达在第 3 天和第 7 天分别显著增加了 9.15 倍和 16.6 倍。在 TGF-β1 处理的 BUMPT 细胞中，circRNA_30032 的表达通过激活 p38 丝裂原活化蛋白激酶信号通路而被诱导。实时定量 PCR、western blot 和双荧光素酶报告基因检测显示，circRNA_30032 通过海绵吸附 miR-96-5p 并增加致纤维化蛋白的表达，包括 HBEGF、KRAS、胶原 I、胶原 III 和纤维连接蛋白，介导 TGF-β1 诱导的和 UUO 诱导的肾纤维化。circRNA_30032 沉默通过增加 miR-96-5p 水平和降低 HBEGF 和 KRAS 水平，显著减少 UUO 模型小鼠的肾纤维化。这些结果表明，circRNA_30032 通过 miR-96-5p/HBEGF/KRAS 轴促进肾纤维化，并表明 circRNA_30032 是治疗肾纤维化的潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/338ae9248123/aging-13-202947-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/93f3f4c9f556/aging-13-202947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/d620f4b8165c/aging-13-202947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/8be8b28aff8c/aging-13-202947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/18ae1902ca8a/aging-13-202947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/43ff72ac3735/aging-13-202947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/7044a6a33377/aging-13-202947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/817dfb3201f1/aging-13-202947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/1363a038bc7c/aging-13-202947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/d9af616b0b0e/aging-13-202947-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/5009fa8b2192/aging-13-202947-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/338ae9248123/aging-13-202947-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/93f3f4c9f556/aging-13-202947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/d620f4b8165c/aging-13-202947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/8be8b28aff8c/aging-13-202947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/18ae1902ca8a/aging-13-202947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/43ff72ac3735/aging-13-202947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/7044a6a33377/aging-13-202947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/817dfb3201f1/aging-13-202947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/1363a038bc7c/aging-13-202947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/d9af616b0b0e/aging-13-202947-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/5009fa8b2192/aging-13-202947-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee0e/8148471/338ae9248123/aging-13-202947-g011.jpg

相似文献

1
CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis.环状 RNA_30032 通过 miRNA-96-5p/HBEGF/KRAS 轴促进 UUO 模型小鼠的肾纤维化。
Aging (Albany NY). 2021 May 11;13(9):12780-12799. doi: 10.18632/aging.202947.
2
The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis.该 mmu_circRNA_37492/hsa_circ_0012138 通过作为 ceRNA 发挥作用，从而减轻梗阻性肾纤维化。
Cell Death Dis. 2022 Mar 4;13(3):207. doi: 10.1038/s41419-022-04612-3.
3
CircRNA_0017076 acts as a sponge for miR-185-5p in the control of epithelial-to-mesenchymal transition of tubular epithelial cells during renal interstitial fibrosis.环状 RNA_0017076 通过海绵吸附 miR-185-5p 调控肾小管上皮细胞 EMT 参与肾间质纤维化。
Hum Cell. 2023 May;36(3):1024-1040. doi: 10.1007/s13577-023-00877-8. Epub 2023 Feb 24.
4
CircRNA_33702 Promotes Renal Fibrosis by Targeting the miR-29b-3p/WNT1-Inducible Signaling Pathway Protein 1 Pathway.环状RNA_33702通过靶向miR-29b-3p/ WNT1诱导信号通路蛋白1途径促进肾纤维化。
J Pharmacol Exp Ther. 2023 Jan;384(1):61-71. doi: 10.1124/jpet.122.001280. Epub 2022 Sep 24.
5
MicroRNA-26a-5p Restoration Ameliorates Unilateral Ureteral Obstruction-Induced Renal Fibrosis in Mice Through Modulating TGF-β Signaling.微小 RNA-26a-5p 的恢复通过调节 TGF-β 信号改善单侧输尿管梗阻诱导的小鼠肾纤维化。
Lab Invest. 2023 Jul;103(7):100131. doi: 10.1016/j.labinv.2023.100131. Epub 2023 Mar 21.
6
Lack of microRNA-155 ameliorates renal fibrosis by targeting PDE3A/TGF-β1/Smad signaling in mice with obstructive nephropathy.miR-155 缺失通过靶向 PDE3A/TGF-β1/Smad 信号通路减轻梗阻性肾病小鼠的肾纤维化。
Cell Biol Int. 2018 Nov;42(11):1523-1532. doi: 10.1002/cbin.11038. Epub 2018 Sep 14.
7
miR-542-5p targets GREM1 to affect the progression of renal fibrosis.miR-542-5p 通过靶向 GREM1 影响肾纤维化的进展。
J Biochem Mol Toxicol. 2024 Sep;38(9):e23818. doi: 10.1002/jbt.23818.
8
Pirfenidone suppresses MAPK signalling pathway to reverse epithelial-mesenchymal transition and renal fibrosis.吡非尼酮可抑制丝裂原活化蛋白激酶信号通路，以逆转上皮-间质转化和肾纤维化。
Nephrology (Carlton). 2017 Aug;22(8):589-597. doi: 10.1111/nep.12831.
9
Rhubarb and Astragalus Capsule Attenuates Renal Interstitial Fibrosis in Rats with Unilateral Ureteral Obstruction by Alleviating Apoptosis through Regulating Transforming Growth Factor beta1 (TGF-β1)/p38 Mitogen-Activated Protein Kinases (p38 MAPK) Pathway.大黄和黄芪胶囊通过调节转化生长因子 β1（TGF-β1）/p38 丝裂原活化蛋白激酶（p38 MAPK）通路减轻细胞凋亡来减轻单侧输尿管梗阻大鼠肾间质纤维化。
Med Sci Monit. 2020 Mar 24;26:e920720. doi: 10.12659/MSM.920720.
10
Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys.管状细胞来源的外泌体 miR-21 通过靶向 PTEN 激活梗阻肾脏中的成纤维细胞促进肾纤维化。
Theranostics. 2021 Aug 2;11(18):8660-8673. doi: 10.7150/thno.62820. eCollection 2021.

引用本文的文献

1
PRDM16 acts as a homeostasis regulation factor to suppress the transition of AKI to CKD via upregulation of eukaryotic initiation factor 6.PRDM16作为一种稳态调节因子，通过上调真核生物起始因子6来抑制急性肾损伤向慢性肾病的转变。
Cell Mol Life Sci. 2025 Jun 23;82(1):252. doi: 10.1007/s00018-025-05766-x.
2
Development of a spontaneous model of renal interstitial fibrosis in NOD/SCID mice: Aging-induced pathogenesis.NOD/SCID小鼠肾间质纤维化自发模型的建立：衰老诱导的发病机制。
PLoS One. 2024 Dec 11;19(12):e0315437. doi: 10.1371/journal.pone.0315437. eCollection 2024.
3
CircInpp5b Ameliorates Renal Interstitial Fibrosis by Promoting the Lysosomal Degradation of DDX1.

本文引用的文献

1
Construction of circRNA-based ceRNA network to reveal the role of circRNAs in the progression and prognosis of metastatic clear cell renal cell carcinoma.构建基于 circRNA 的 ceRNA 网络，揭示 circRNAs 在转移性透明细胞肾细胞癌进展和预后中的作用。
Aging (Albany NY). 2020 Nov 20;12(23):24184-24207. doi: 10.18632/aging.104107.
2
CircPDZD8 promotes gastric cancer progression by regulating CHD9 via sponging miR-197-5p.环状PDZD8通过海绵化miR-197-5p调控CHD9促进胃癌进展。
Aging (Albany NY). 2020 Oct 13;12(19):19352-19364. doi: 10.18632/aging.103805.
3
Circular RNA in renal diseases.
环状 RNA 内肽酶 5b 通过促进 DDX1 的溶酶体降解来改善肾间质纤维化。
Biomolecules. 2024 May 23;14(6):613. doi: 10.3390/biom14060613.
4
RfxCas13d-mediated inhibition of alleviates renal fibrosis via PI3K/AKT signaling pathway.RfxCas13d 介导的抑制作用通过 PI3K/AKT 信号通路减轻肾纤维化。
Ren Fail. 2024 Dec;46(1):2331612. doi: 10.1080/0886022X.2024.2331612. Epub 2024 Mar 25.
5
Anti-Liver Fibrosis Role of miRNA-96-5p via Targeting FN1 and Inhibiting ECM-Receptor Interaction Pathway.miRNA-96-5p 通过靶向 FN1 并抑制 ECM-受体相互作用通路发挥抗肝纤维化作用。
Appl Biochem Biotechnol. 2023 Nov;195(11):6840-6855. doi: 10.1007/s12010-023-04385-1. Epub 2023 Mar 21.
6
Identification of an unrecognized circRNA associated with development of renal fibrosis.鉴定一种与肾纤维化发展相关的未被识别的环状RNA。
Front Genet. 2023 Jan 4;13:964840. doi: 10.3389/fgene.2022.964840. eCollection 2022.
7
LncRNA 148400 Promotes the Apoptosis of Renal Tubular Epithelial Cells in Ischemic AKI by Targeting the miR-10b-3p/GRK4 Axis.LncRNA 148400 通过靶向 miR-10b-3p/GRK4 轴促进缺血性 AKI 中肾小管上皮细胞的凋亡。
Cells. 2022 Dec 9;11(24):3986. doi: 10.3390/cells11243986.
8
Circular RNAs in Acute Kidney Injury: Roles in Pathophysiology and Implications for Clinical Management.环状 RNA 在急性肾损伤中的作用：在病理生理学中的作用及其对临床管理的影响。
Int J Mol Sci. 2022 Jul 31;23(15):8509. doi: 10.3390/ijms23158509.
9
mmu-lncRNA 121686/hsa-lncRNA 520657 induced by METTL3 drive the progression of AKI by targeting miR-328-5p/HtrA3 signaling axis.METTL3 诱导的 mmu-lncRNA 121686/hsa-lncRNA 520657 通过靶向 miR-328-5p/HtrA3 信号轴促进 AKI 的进展。
Mol Ther. 2022 Dec 7;30(12):3694-3713. doi: 10.1016/j.ymthe.2022.07.014. Epub 2022 Jul 21.
10
Urinary exosomes derived circRNAs as biomarkers for chronic renal fibrosis.尿液外泌体来源的 circRNAs 作为慢性肾纤维化的生物标志物。
Ann Med. 2022 Dec;54(1):1966-1976. doi: 10.1080/07853890.2022.2098374.
环状 RNA 在肾脏疾病中的作用。
J Cell Mol Med. 2020 Jun;24(12):6523-6533. doi: 10.1111/jcmm.15295. Epub 2020 Apr 25.
4
Circular RNA YAP1 acts as the sponge of microRNA-21-5p to secure HK-2 cells from ischaemia/reperfusion-induced injury.环状 RNA YAP1 作为 microRNA-21-5p 的海绵，保护 HK-2 细胞免受缺血/再灌注损伤。
J Cell Mol Med. 2020 Apr;24(8):4707-4715. doi: 10.1111/jcmm.15142. Epub 2020 Mar 11.
5
circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis.环状 RNA ACTR2：通过细胞焦亡调控高糖诱导的肾小管细胞纤维化的新机制
Biol Pharm Bull. 2020;43(3):558-564. doi: 10.1248/bpb.b19-00901.
6
The Biomarker TCONS_00016233 Drives Septic AKI by Targeting the miR-22-3p/AIFM1 Signaling Axis.生物标志物TCONS_00016233通过靶向miR-22-3p/AIFM1信号轴驱动脓毒症急性肾损伤。
Mol Ther Nucleic Acids. 2020 Mar 6;19:1027-1042. doi: 10.1016/j.omtn.2019.12.037. Epub 2020 Jan 14.
7
LncRNA GAS5 exacerbates renal tubular epithelial fibrosis by acting as a competing endogenous RNA of miR-96-5p.长链非编码 RNA GAS5 通过作为 miR-96-5p 的竞争性内源性 RNA 加剧肾小管上皮细胞纤维化。
Biomed Pharmacother. 2020 Jan;121:109411. doi: 10.1016/j.biopha.2019.109411. Epub 2019 Nov 21.
8
Circ_0080425 inhibits cell proliferation and fibrosis in diabetic nephropathy via sponging miR-24-3p and targeting fibroblast growth factor 11.环状 RNA 0080425 通过海绵吸附 miR-24-3p 并靶向成纤维细胞生长因子 11 抑制糖尿病肾病中的细胞增殖和纤维化。
J Cell Physiol. 2020 May;235(5):4520-4529. doi: 10.1002/jcp.29329. Epub 2019 Nov 3.
9
Inhibition of Kirsten-Ras reduces fibrosis and protects against renal dysfunction in a mouse model of chronic folic acid nephropathy.Kirsten-Ras 抑制可减少纤维化并防止慢性叶酸肾病小鼠模型的肾功能障碍。
Sci Rep. 2019 Sep 30;9(1):14010. doi: 10.1038/s41598-019-50422-7.
10
Circular RNA CircFndc3b modulates cardiac repair after myocardial infarction via FUS/VEGF-A axis.环状 RNA CircFndc3b 通过 FUS/VEGF-A 轴调节心肌梗死后的心脏修复。
Nat Commun. 2019 Sep 20;10(1):4317. doi: 10.1038/s41467-019-11777-7.