Spadea Leopoldo, Zanotto Elisa, Cavallo Rossana, Campagna Giuseppe, Giannico Maria Ilaria, Costagliola Ciro
From the Eye Clinic, Policlinico Umberto I, "Sapienza" University of Rome, Rome, Italy (Spadea, Giannico); Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy (Zanotto, Cavallo); Department of Medical-Surgical Sciences and Translational Medicine, "Sapienza" University of Rome, Rome, Italy (Campagna); Department of Medicine and Health Science "V. Tiberio," University of Molise, Campobasso, Italy (Costagliola).
J Cataract Refract Surg. 2021 Dec 1;47(12):1548-1555. doi: 10.1097/j.jcrs.0000000000000672.
To evaluate the antimicrobial effectiveness of a liposomal ozonized oil solution used as a home therapy in patients undergoing cataract surgery. Antimicrobial efficacy was evaluated as the reduction in the bacterial load of the most common bacteria isolated from cases with endophthalmitis.
20 Italian experimental centers of the Effectiveness of Liposomal Ozonized oil on Ocular Microbial flora before cataract surgery study group.
Interventional, nonrandomized, paired-eye designed, phase 4 clinical study.
A total of 174 patients undergoing cataract surgery were divided into 2 groups: the study group (174 eyes) underwent surgery and received an isotonic ophthalmic solution of 0.5% ozonized oil in liposomes plus hypromellose treatment (2 drops 4 times/d), and the control group (174 contralateral eyes) was treated with saline solution. The treatment lasted for 3 days. Subconjunctival swabs were taken from both eyes of each patient at T0 (the day before starting the treatment and 4 days preoperatively) and at T4 (after 3 days of treatment and 10 min preoperatively) and sent to the laboratory within 24 hours of collection for microbiological analysis.
30% of 696 swabs taken at T0 were sterile. Contaminated swabs had a high prevalence of coagulase-negative staphylococci, including Staphylococcus epidermidis, and more than 60 different bacterial species were isolated. A significant reduction in microbial load was observed after treatment (>90% of the samples). The microbial load in the control group remained unchanged.
Liposomal ozonized oil reduced the microbial burden after topical administration in a large study population.
评估脂质体臭氧油溶液作为家庭疗法用于白内障手术患者时的抗菌效果。抗菌效果通过眼内炎病例中分离出的最常见细菌的细菌载量减少来评估。
意大利20个实验中心参与白内障手术前脂质体臭氧油对眼部微生物菌群有效性研究组。
干预性、非随机、双眼配对设计的4期临床研究。
总共174例接受白内障手术的患者被分为2组：研究组（174只眼）接受手术并接受脂质体中0.5%臭氧油加羟丙甲纤维素的等渗眼用溶液治疗（每日4次，每次2滴），对照组（174只对侧眼）用盐溶液治疗。治疗持续3天。在T0（开始治疗前一天及术前4天）和T4（治疗3天后及术前10分钟）从每位患者的双眼采集结膜下拭子，并在采集后24小时内送至实验室进行微生物分析。
在T0采集的696份拭子中，30%无菌。受污染的拭子中凝固酶阴性葡萄球菌（包括表皮葡萄球菌）的患病率很高，并且分离出了60多种不同的细菌种类。治疗后观察到微生物载量显著降低（>90%的样本）。对照组中的微生物载量保持不变。
在一项大型研究人群中，局部应用脂质体臭氧油可降低微生物负荷。
