Department of Obstetrics and Gynaecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China.
Gynecol Endocrinol. 2021 Aug;37(8):725-729. doi: 10.1080/09513590.2021.1918663. Epub 2021 May 11.
Study the effect of low-dose aspirin on the endometrial receptivity in endometriosis rat models.
This study is to explore the expressions of progesterone receptor and LIF among three groups of endometriosis rat models: control group ( = 12), EMs group ( = 15), and aspirin group ( = 17). The expressions of progesterone receptor (PR), PRA, PRB, and leukemia inhibitory factor receptor (LIFR) in eutopic endometrium were determined using immunohistochemistry technology, western blot, and qRT-PCR. The levels of LIF in eutopic endometrium and serum were detected by western blot, qRT-PCR, and ELISA.
The expressions of PR, PRA, and PRB protein were significantly increased in the eutopic endometrium after low-dose aspirin treatment, and the level of PRB mRNA was also increased while the ratio of PRA/PRB mRNA was decreased in the eutopic endometrium. The levels of LIF in eutopic endometrium and serum were increased compared with the untreated endometriosis rats. However, the expression of LIFR was not statistically different among the three groups.
The results suggest that the low-dose aspirin treatment could downregulate progesterone resistance and increase the expression of LIF of endometriosis rats during the implantation window, which could improve endometrial receptivity and enhance the pregnant rate of endometriosis. It may provide a potential treatment method for endometriosis-related infertility.
研究小剂量阿司匹林对子宫内膜异位症大鼠模型子宫内膜容受性的影响。
本研究旨在探讨三组子宫内膜异位症大鼠模型（对照组 n=12，子宫内膜异位症组 n=15，阿司匹林组 n=17）中孕激素受体和 LIF 的表达情况。采用免疫组织化学技术、Western blot 和 qRT-PCR 检测在位子宫内膜中孕激素受体（PR）、PRA、PRB 和白血病抑制因子受体（LIFR）的表达。采用 Western blot、qRT-PCR 和 ELISA 检测在位子宫内膜和血清中 LIF 的水平。
低剂量阿司匹林治疗后，在位子宫内膜中 PR、PRA 和 PRB 蛋白的表达明显增加，在位子宫内膜中 PRB mRNA 的水平也增加，而 PRA/PRB mRNA 的比值降低。与未经处理的子宫内膜异位症大鼠相比，在位子宫内膜和血清中的 LIF 水平升高。然而，三组之间 LIFR 的表达没有统计学差异。
结果表明，低剂量阿司匹林治疗可下调孕激素抵抗并增加子宫内膜异位症大鼠着床窗期间 LIF 的表达，从而改善子宫内膜容受性，提高子宫内膜异位症的妊娠率。它可能为子宫内膜异位症相关不孕提供一种潜在的治疗方法。
