Liu Guan, Yu Xia, Luo Jingjing, Hu Yanjie, Dong Lingling, Jiang Guanglu, Huo Fengmin, Wang Fen, Liang Qian, Huang Hairong
Wuhan Pulmonary Hospital, Wuhan Institution of Tuberculosis Control, Wuhan 430030, PR China.
National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, PR China.
Int J Syst Evol Microbiol. 2021 May;71(5). doi: 10.1099/ijsem.0.004796.
A slow-growing, scotochromogenic mycobacterial strain (24) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24 to the complex, which includes and . The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on and sequences strongly supported the taxonomic status of strain 24 as a representative of a species distinct from the other members of the complex. The genomic G+C content of strain 24 was 65.40mol%. Genomic comparisons showed that strain 24 and ATCC 14470 had an average nucleotide identity (ANI) value of 81.00 % and a DNA-DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24and 49 061 were 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24 is distinguishable from its closest phylogenetic relatives and represents a novel species of the genus , therefore the name sp. nov. is proposed. The type strain is 24 (=CMCC 93559=DSM 105979).
从一名中国男性痰液中分离出一株生长缓慢的暗产色分枝杆菌菌株（24）。使用16S rRNA基因进行的系统发育分析将菌株24归入该复合体，该复合体包括[具体名称1]和[具体名称2]。基于16S rRNA基因、hsp65基因和rpoB基因序列的表型特征、独特的分枝菌酸谱以及系统发育分析结果，有力地支持了菌株24作为不同于该复合体其他成员的一个物种代表的分类地位。菌株24的基因组G+C含量为65.40mol%。基因组比较显示，菌株24与[菌株名称1]ATCC 14470的平均核苷酸同一性（ANI）值为81.00%，DNA-DNA杂交（DDH）值为22.80%，而菌株24与[菌株名称2]49061之间的ANI和DDH值分别为80.98和22.80%。从系统发育、表型和化学分类学特征来看，菌株24与其最接近的系统发育亲缘种不同，代表了该属的一个新物种，因此提出新物种名称[新物种名称]sp. nov.。模式菌株为24（=CMCC 93559=DSM 105979）。
