Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba, Higashi-Murayama, Tokyo, Japan.
SRL Inc, 50 Fuchigami, Akiruno, Tokyo, Japan.
Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005917.
Scotochromogenic slow-growing mycobacteria were isolated from the sputum or bronchoalveolar lavage fluid of 12 patients in Japan. From a comparison of the whole-genome sequences, the representative strain IWGMT90018-18076 and the unknown strains obtained from the patients were found to represent a novel species related to the complex. The average nucleotide identity values of IWGMT90018-18076 with , and were 86.7, 82.5 and 82.2 %, respectively. The genome size of the representative strain IWGMT90018-18076 was approximately 6.3 Mbp, and the genomic DNA G+C content was 67.1 %. The major fatty acid methyl esters were C (37.71 %), Cω9 (29.5 %) and Cω7 (10.32 %). In this study, we performed phylogenetic analyses, physiological and biochemical characteristic tests, drug susceptibility tests and fatty acid profiling of the clinical isolates. On the basis of the results obtained, we propose that the unknown clinical isolates represent a novel species, ' sp. nov,' with the type strain being IWGMT90018-18076 (=JCM 34837 =KCTC 49725).
从日本 12 名患者的痰液或支气管肺泡灌洗液中分离出了产色缓慢生长分枝杆菌。通过比较全基因组序列，发现代表菌株 IWGMT90018-18076 和从患者中获得的未知菌株代表了与 复合体相关的新型物种。IWGMT90018-18076 与 、 和 的平均核苷酸同一性值分别为 86.7、82.5 和 82.2%。代表菌株 IWGMT90018-18076 的基因组大小约为 6.3 Mbp，基因组 DNA G+C 含量为 67.1%。主要脂肪酸甲酯为 C（37.71%）、Cω9（29.5%）和 Cω7（10.32%）。在这项研究中，我们对临床分离株进行了系统发育分析、生理生化特性测试、药敏试验和脂肪酸图谱分析。根据获得的结果，我们建议这些未知的临床分离株代表了一个新的物种，“ sp. nov. ”，其模式菌株为 IWGMT90018-18076（=JCM 34837 =KCTC 49725）。
