Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health and Internet Technology, College of Animal Science and Technology, Zhejiang Agriculture & Forestry University, Hangzhou 311300, China.
College of Animal Science, Zhejiang University, Hangzhou 310058, China.
Poult Sci. 2021 Jun;100(6):101168. doi: 10.1016/j.psj.2021.101168. Epub 2021 Mar 27.
This study evaluated the effects of Bacillus coagulans (B. coagulans) as an alternative to antibiotics on growth performance, antioxidant capacity, immunity function and gut health in broilers. A total of 480 one-day-old broilers were randomly divided into 3 treatments with 8 replicates comprising 20 broilers each. The experiment lasted 42 d. Treatments included: basal diet without antibiotics (NCO); basal diet supplemented with 75 mg/kg chlortetracycline (ANT); basal diet supplemented with 5 × 10 CFU/kg B. coagulans(BC). The B. coagulans enhanced body weight (BW) and average daily gain compared with the NCO group (P < 0.05). However, there were no significant differences in average daily feed intake and feed: gain ratio (F: G) among three groups (P > 0.05). The B. coagulans significantly increased catalase, superoxide dismutase, and glutathione peroxidase levels and reduced malondialdehyde levels (P < 0.05). The serum immunoglobulins (IgA, IgM, and IgY) were significantly higher in the BC group when compared to the NCO and ANT groups (P < 0.05). The B. coagulans also markedly reduced serum levels of proinflammatory factors (IL-1β, IL-6, and TNF-α) and enhanced anti-inflammatory factor (IL-10) concentrations compared with control group (P < 0.05). Moreover, compared with the control group, BC significantly inhibited serum xanthine oxidase activity (P < 0.05). The levels of acetic acid, propionic acid, butyrate, isobutyric acid and valerate in BC group were significantly increased on d 42 compared with the NCO and ANT groups (P < 0.05). Furthermore, BC significantly altered cecal microbiota by reducing Desulfovibrio and Parasutterella, and by increasing Alistipes and Odoribacter (P < 0.05, P < 0.05, P < 0.001, P < 0.01, respectively). In conclusion, dietary B. coagulans, when used as an alternative to antibiotics, improved body weight, average daily gain, antioxidant capacity, immunity function and gut health in broilers.
本研究评估了凝结芽孢杆菌（B. coagulans）作为抗生素替代品对肉鸡生长性能、抗氧化能力、免疫功能和肠道健康的影响。将 480 只 1 日龄肉鸡随机分为 3 个处理组，每组 8 个重复，每个重复 20 只鸡。试验持续 42 天。处理组包括：不含抗生素的基础日粮（NCO）；基础日粮添加 75mg/kg 金霉素（ANT）；基础日粮添加 5×10CFU/kg 凝结芽孢杆菌（BC）。与 NCO 组相比，凝结芽孢杆菌组的体重（BW）和平均日增重（ADG）更高（P＜0.05）。然而，三组的平均日采食量和饲料：增重比（F:G）均无显著差异（P＞0.05）。凝结芽孢杆菌组的过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶水平显著升高，丙二醛水平显著降低（P＜0.05）。与 NCO 和 ANT 组相比，BC 组血清免疫球蛋白（IgA、IgM 和 IgY）显著升高（P＜0.05）。凝结芽孢杆菌还显著降低了血清促炎因子（IL-1β、IL-6 和 TNF-α）水平，提高了抗炎因子（IL-10）浓度，与对照组相比（P＜0.05）。此外，与对照组相比，BC 显著抑制了血清黄嘌呤氧化酶的活性（P＜0.05）。BC 组在第 42 天的乙酸、丙酸、丁酸、异丁酸和戊酸水平均显著高于 NCO 和 ANT 组（P＜0.05）。此外，BC 通过降低脱硫弧菌和副拟杆菌，增加拟杆菌和恶臭杆菌，显著改变了盲肠微生物群（P＜0.05，P＜0.05，P＜0.001，P＜0.01，分别）。综上所述，日粮凝结芽孢杆菌作为抗生素替代品，可提高肉鸡的体重、平均日增重、抗氧化能力、免疫功能和肠道健康。
