Ma Li, Tian Guangming, Pu Yuejin, Qin Xuguang, Zhang Yinghu, Wang Haojie, You Lei, Zhang Gaofeng, Fang Chun, Liang Xiongyan, Wei Hongbo, Tan Lei, Jiang Liren
College of Animal Science and Technology, Yangtze University, Jingzhou, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.
Front Microbiol. 2024 Nov 29;15:1492035. doi: 10.3389/fmicb.2024.1492035. eCollection 2024.
This study aimed to assess the protective efficacy of MF-06 as a potential alternative to antibiotics in mitigating intestinal mucosal damage in chicks infected with .
A total of 150 one-day-old SPF chicks were selected and randomly divided into five groups: control group (CK), probiotics group (EM), probiotics treatment group (PT), antibiotic treatment group (AT), group (SI), CK, AT and SI groups were fed a basal diet, EM and PT groups were fed a basal diet supplemented with 1.0 × 10 CFU/g ; PT, AT and SI groups were gavaged with 1.0 × 10 CFU/0.5 mL at 7 days of age; AT group were fed with 0.375 g/kg neomycin sulfate in the basal diet from days 7-14.
Subsequently, the study evaluated alterations in growth performance, the integrity of the intestinal mucosal barrier, cytokines associated with the Wnt/-catenin signaling pathway, and gut microbiota composition. The results revealed that the administration of MF-06 significantly reduced the feed conversion ratio of chicks ( < 0.05), and significantly increased the average daily weight gain and average daily feed intake in chicks challenged with ( < 0.05). Furthermore, MF-06 treatment diminished the presence of colonies in the intestinal tract. Additionally, the administration of MF-06 restored levels of (Diamine oxidase) DAO and (D-lactic acid) D-LA levels, as well as the levels of tight junction protein, including , , , , and ( < 0.05). The study noted a significant decrease in cell apoptosis ( < 0.05) and a significant increase in the expression of Proliferating Cell Nuclear Antigen (PCNA) and v-myc avian myelocytomatosis viral oncogene homolog (C-MYC) ( < 0.05), which activated the Wnt/-catenin signaling pathway. Analysis through 16S rRNA sequencing revealed that the intake of MF-06 led to a significant decrease in the relative abundance of , , and ( < 0.05).
Collectively, the MF-06 may provide a protective effect against infection in chicks by enhancing growth performance, strengthening the integrity of the intestinal mucosal barrier, and stabilizing the gut microbiota.
本研究旨在评估MF-06作为抗生素的潜在替代品在减轻感染鸡肠道黏膜损伤方面的保护效果。
选取150只1日龄SPF鸡，随机分为五组：对照组（CK）、益生菌组（EM）、益生菌处理组（PT）、抗生素处理组（AT）、感染组（SI）。CK、AT和SI组饲喂基础日粮，EM和PT组饲喂添加1.0×10⁸CFU/g的基础日粮；PT、AT和SI组在7日龄时灌胃1.0×10⁸CFU/0.5 mL；AT组在7-14日龄期间在基础日粮中添加0.375 g/kg硫酸新霉素。
随后，该研究评估了生长性能、肠道黏膜屏障完整性、与Wnt/β-连环蛋白信号通路相关的细胞因子以及肠道微生物群组成的变化。结果显示，给予MF-06显著降低了鸡的料重比（P<0.05），并显著提高了感染鸡的平均日增重和平均日采食量（P<0.05）。此外，MF-06处理减少了肠道中菌落的存在。此外，给予MF-06恢复了二胺氧化酶（DAO）和D-乳酸（D-LA）水平，以及紧密连接蛋白水平，包括Claudin-1、Occludin、ZO-1、ZO-2和E-cadherin（P<0.05）。该研究指出细胞凋亡显著减少（P<0.05），增殖细胞核抗原（PCNA）和v-myc禽骨髓细胞瘤病毒癌基因同源物（C-MYC）的表达显著增加（P<0.05），从而激活了Wnt/β-连环蛋白信号通路。通过16S rRNA测序分析表明，摄入MF-06导致大肠杆菌、肠球菌和沙门氏菌的相对丰度显著降低（P<0.05）。
总体而言，MF-06可能通过提高生长性能、增强肠道黏膜屏障完整性和稳定肠道微生物群，对鸡感染提供保护作用。
