International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan; Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Res Vet Sci. 2021 Jul;137:138-143. doi: 10.1016/j.rvsc.2021.05.002. Epub 2021 May 4.
Pasteurella multocida (P. multocida) infects the swine respiratory tract and mainly causes atrophic rhinitis (AR). Recently, many commercially inactivated and subunit vaccines have been used as preventive strategies. However, the best antigenic protein portion has not been selected, and the aluminum gel was used as the adjuvant, which may not induce full protection. P. multocida toxin (PMT) is the major virulence factor responsible for AR. PMT is a monomeric 146 kDa protein (approximately 1285 amino acids) encoded by the tox A gene. In this study, we expressed different fragments of recombinant PMT proteins, combined them with a water-in-oil-in-water adjuvant, and evaluated mice's immune response. The results indicated that the rPMT-C-immunized group showed significantly higher levels (p < 0.05) of IgG, IgG2a antibody and interferon-γ, IL-12 cytokine expression than other groups. Furthermore, vaccination with rPMT-C recombinant protein can provide homologous and heterologous protection against P. multocida challenge. In conclusion, our approach may be feasible for developing an effective subunit vaccine against atrophic rhinitis with a cost-down simple ingredient.
多杀巴斯德菌（Pasteurella multocida，P. multocida）感染猪的呼吸道，主要引起萎缩性鼻炎（atrophic rhinitis，AR）。目前已有许多商业上使用的灭活疫苗和亚单位疫苗被作为预防策略。然而，最佳抗原蛋白部分尚未被选择，并且使用了铝凝胶作为佐剂，这可能无法诱导完全保护。多杀巴斯德菌毒素（Pasteurella multocida toxin，PMT）是导致 AR 的主要毒力因子。PMT 是一种单体 146 kDa 蛋白（约 1285 个氨基酸），由 tox A 基因编码。在本研究中，我们表达了重组 PMT 蛋白的不同片段，将其与水包油包水佐剂结合，并评估了小鼠的免疫反应。结果表明，rPMT-C 免疫组的 IgG、IgG2a 抗体和干扰素-γ、IL-12 细胞因子表达水平明显高于其他组（p<0.05）。此外，rPMT-C 重组蛋白疫苗接种可提供针对 P. multocida 挑战的同源和异源保护。总之，我们的方法可能是可行的，可用于开发具有成本效益的简单成分的萎缩性鼻炎有效亚单位疫苗。
