Research & Development Department, Research & Development Section, Meiji Animal Health Co., Ltd., Kumamoto, Japan.
PLoS One. 2024 May 20;19(5):e0301688. doi: 10.1371/journal.pone.0301688. eCollection 2024.
Swine atrophic rhinitis is a disease caused by Pasteurella multocida and Bordetella bronchiseptica that affects pigs. Inactivated vaccines containing the toxins produced by Pasteurella multocida and Bordetella bronchiseptica have been widely used for the prevention of swine atrophic rhinitis. The efficacy of a vaccine is correlated with the amount of antigen present; however, the protective toxin of P. multocida bound to aluminum hydroxide, which is used as an adjuvant, can hinder the monitoring of the antigen concentration in the vaccine. This study assessed the applicability of a dot immunoassay as an antigen quantification method using monoclonal antibodies. This quantification method was able to detect the antigen with high specificity and sensitivity even when the antigen was bound to the adjuvant, and its application to vaccine products revealed a correlation between the amount of antigen present in the vaccine and the neutralizing antibody titers induced in pigs. The antigen quantification method presented in this study is a simple and sensitive assay capable of quantifying the amount of antigen present in a vaccine that can be used as an alternative quality control measure.
猪萎缩性鼻炎是由多杀性巴氏杆菌和支气管败血波氏杆菌引起的一种疾病，影响猪。含有多杀性巴氏杆菌和支气管败血波氏杆菌产生的毒素的灭活疫苗已被广泛用于预防猪萎缩性鼻炎。疫苗的功效与存在的抗原量相关；然而，与用作佐剂的氢氧化铝结合的多杀性巴氏杆菌的保护性毒素可以阻碍疫苗中抗原浓度的监测。本研究评估了使用单克隆抗体的斑点免疫分析作为抗原定量方法的适用性。即使抗原与佐剂结合，这种定量方法也能够以高特异性和灵敏度检测抗原，并且其在疫苗产品中的应用表明疫苗中存在的抗原量与诱导猪体内中和抗体滴度之间存在相关性。本研究提出的抗原定量方法是一种简单、敏感的检测方法，能够定量检测疫苗中的抗原含量，可作为一种替代的质量控制措施。
