Seo Jayoung, Pyo Hyoju, Lee Semi, Lee Jaeil, Kim Taejung
Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Can J Vet Res. 2009 Jul;73(3):184-9.
Pasteurella multocida toxin (PMT) is a poor antigen that becomes more immunogenic after its native structure has been destroyed. In contrast, partially truncated PMT proteins, which are predicted to be good antigens when used as a vaccine, might be used to improve the control of atrophic rhinitis in pigs. In this study, 4 truncated PMT fragments were expressed in Escherichia coli, and those 4 fragments were inoculated into mice to produce the polyclonal antibodies. The results of an enzyme-linked immunosorbent assay (ELISA) revealed that #1 and #4 fragments were the most immunogenic. Immunized mice were subsequently challenged intraperitoneally with P. multocida type D. Five of the eight #1 fragment-immunized mice showed some protection against death and bacterial clearance. Pigs immunized with #1 fragment produced no or mild atrophic rhinitis (turbinate conchal score) after challenge, suggesting that this #1 fragment could be a good candidate for a subunit recombinant-type vaccine.
多杀性巴氏杆菌毒素（PMT）是一种较差的抗原，在其天然结构被破坏后免疫原性会增强。相比之下，部分截短的PMT蛋白在用作疫苗时预计是良好的抗原，可能用于改善猪萎缩性鼻炎的防控。在本研究中，4个截短的PMT片段在大肠杆菌中表达，并将这4个片段接种到小鼠体内以产生多克隆抗体。酶联免疫吸附测定（ELISA）结果显示，#1和#4片段免疫原性最强。随后，用D型多杀性巴氏杆菌对免疫的小鼠进行腹腔攻毒。在8只接种#1片段的小鼠中，有5只表现出对死亡和细菌清除的一定保护作用。用#1片段免疫的猪在攻毒后未出现或仅出现轻度萎缩性鼻炎（鼻甲贝壳状评分），表明该#1片段可能是亚单位重组型疫苗的良好候选物。
