Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Coquimbo, Chile; Laboratorio de Ecología y Conducta de La Ontogenia Temprana (LECOT), Coquimbo, Chile.
Departamento de Biología Marina, Facultad de Ciencias Del Mar, Universidad Católica Del Norte, Larrondo 1281, Coquimbo, Chile; Centro de Investigación y Desarrollo Tecnológico en Algas y Otros Recursos Biológicos (CIDTA), Facultad de Ciencias Del Mar, Universidad Católica Del Norte, Larrondo 1281, Coquimbo, Chile.
Environ Pollut. 2021 Oct 1;286:117224. doi: 10.1016/j.envpol.2021.117224. Epub 2021 May 4.
Understanding the impact of increases in pCO (OA) and extreme changes in temperature on marine organisms is critical to predicting how they will cope with climate change. We evaluated the effects of OA as well as warming and cooling trend temperature on early reproductive traits of Lessonia trabeculata, a bio-engineer kelp species. Sori discs were maintained for an exposure time (ET) of 3 (T3) and 7 (T7) days to one of two contrasting pCO levels (450 and 1100 μatm). In addition, at each pCO level, they were subjected to three temperature treatments: 15 °C (control), 10 °C (cool) and 19 °C (warm). Subsequently, we compared sorus photosynthetic performance (Fv/Fm), the number of meiospores released (MR) and their germination rate (GR) after 48 h of settlement, with values obtained from sori discs not exposed (DNE) to the treatments. The Fv/Fm measured for DNE was lower than at T3 and T7 at 10 and 15 °C but not at 19 °C. Regardless of temperature, we found no significant differences between MR measured at T0 and T3. MR at T7 was significantly lower at 19 °C than at 10 and 15 °C. We found only a significant reduction in MR in response to elevated pCO at T3. The GR of meiospores released by DNE and then maintained for 48 h to 19 °C decreased significantly by 33% when compared with those maintained for the same time at 10 and 15 °C. A similar, but more drastic reduction (54%) in the GR was found in meiospores released by sori discs exposed for T3 and maintained for 48 h to 19 °C. We suggest that OA and warming trend will threaten the early establishment of this species with further consequences for the functioning of the associated ecosystem.
了解 pCO（OA）增加和温度极端变化对海洋生物的影响对于预测它们如何应对气候变化至关重要。我们评估了 OA 以及增温和降温趋势温度对 Lessonia trabeculata 早期生殖特征的影响，Lessonia trabeculata 是一种生物工程褐藻物种。将孢子盘保持在暴露时间 (ET) 3 天（T3）和 7 天（T7），分别暴露于两种对比 pCO 水平（450 和 1100 μatm）之一。此外，在每个 pCO 水平下，它们还受到三种温度处理：15°C（对照）、10°C（冷）和 19°C（暖）。随后，我们比较了暴露于处理的孢子盘（T3 和 T7）的光合作用性能（Fv/Fm）、释放的减数孢子数量（MR）及其在定居后 48 小时的发芽率（GR）与未暴露于处理的孢子盘（DNE）的数值。在 10°C 和 15°C 时，DNE 测量的 Fv/Fm 低于 T3 和 T7，但在 19°C 时则不然。无论温度如何，我们都发现 T0 和 T3 测量的 MR 之间没有显着差异。在 19°C 时，T7 的 MR 显着低于 10°C 和 15°C。我们仅发现 T3 时升高的 pCO 会导致 MR 显着降低。DNE 释放的减数孢子在 19°C 下保持 48 小时后，其发芽率与在 10°C 和 15°C 下保持相同时间相比，显著降低了约 33%。在 T3 下暴露并保持 48 小时后释放的减数孢子的 GR 也发现了类似但更剧烈的降低（~54%）。我们认为，OA 和变暖趋势将威胁到该物种的早期建立，从而对相关生态系统的功能产生进一步影响。
