钩端螺旋体在固体培养基上的溶血活性研究。
Investigation of hemolytic activity of leptospirae on solid culture media.
作者信息
Volina E G, Kiktenko V S, Sarukhanova L E, Levina L F, Arutyunova G A, Soboleva G L
机构信息
Department of Microbiology, Medical Faculty of the Patrice Lumumba University of Friendship of Peoples, Moscow, USSR.
出版信息
J Hyg Epidemiol Microbiol Immunol. 1988;32(1):79-85.
Results of investigation of hemolytic activity of leptospirae against red blood cells of various animal species on solid culture media using the technique of agar layers are presented. It has been shown that hemolytic properties of pathogenic and saprophytic leptospirae differ with respect to red blood cells of the sheep, the rabbit, the hamster, the albino rat and the albino mouse. Hemolytic activity of leptospirae regarding the erythrocytes of the cattle, the horse and the fowl depends on the strain of leptospirae. The advantage of using solid nutrient media for the determination of hemolytic properties of leptospirae in comparison with liquid media has been demonstrated.
本文展示了采用琼脂层技术在固体培养基上对钩端螺旋体针对不同动物物种红细胞的溶血活性进行研究的结果。结果表明，致病性钩端螺旋体和腐生性钩端螺旋体针对绵羊、兔子、仓鼠、白化大鼠和白化小鼠的红细胞的溶血特性有所不同。钩端螺旋体针对牛、马和禽类红细胞的溶血活性取决于钩端螺旋体的菌株。与液体培养基相比，已证明使用固体营养培养基测定钩端螺旋体溶血特性具有优势。
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