Centre of Reproductive Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of Münster, Münster, Germany.
NORDFERTIL Research Lab Stockholm, Department of Women's and Children's Health, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
Fertil Steril. 2021 Sep;116(3):713-720. doi: 10.1016/j.fertnstert.2021.04.019. Epub 2021 May 8.
To normalize age-dependent effects on standardized measures of spermatogonial quantity such as the number of spermatogonia per tubular cross-section (S/T) or fertility index.
Published quantitative histologic data on human spermatogonial numbers were used to create Z-scores for reference means and tested on archived testicular tissue samples.
Retrospective cohort study.
PATIENT(S): The sample cohort comprised testicular samples from 24 boys with cancer diagnosis and 10 with Klinefelter syndrome, as part of the fertility preservation programs NORDFERTIL and Androprotect, as well as archived histologic samples from 35 prepubertal boys with acute lymphoblastic leukemia and 20 testicular biobank samples.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Z-score values for S/T and fertility index on the basis of morphology and germ cell-specific markers (MAGEA4 and/or DDX4) were calculated, and the impact of cancer therapy exposure and genetic disorders on Z-score values was evaluated.
RESULT(S): The Z-scores for S/T values in the nontreated samples (-2.08 ± 2.20, n = 28) and samples treated with nonalkylating agents (-1.90 ± 2.60, n = 25) were comparable within ±3 standard deviations of the reference mean value but differed significantly from samples exposed to alkylating agents (-12.14 ± 9.20, n = 22) and from patients with Klinefelter syndrome (-11.56 ± 4.89, n = 8). The Z-scores for S/T were correlated with increasing cumulative exposure to alkylating agents (r = -0.7020).
CONCLUSION(S): The Z-score values for S/T allow for the quantification of genetic and cancer treatment-related effects on testicular tissue stored for fertility preservation, facilitating their use for patient counseling.
使标准化精子发生数量测量指标（如每横切管精子数[S/T]或生育指数）的年龄依赖性影响正常化。
使用已发表的人类精子发生数量的定量组织学数据创建参考均值的 Z 分数，并在存档的睾丸组织样本上进行测试。
回顾性队列研究。
样本队列包括 24 名癌症诊断男孩和 10 名克氏综合征男孩的睾丸样本，这些样本来自 NORDFERTIL 和 Androprotect 生育保护计划，以及 35 名急性淋巴细胞白血病青春期前男孩和 20 名睾丸生物库样本的存档组织学样本。
无。
根据形态学和生殖细胞特异性标志物（MAGEA4 和/或 DDX4）计算 S/T 和生育指数的 Z 分数值，并评估癌症治疗暴露和遗传疾病对 Z 分数值的影响。
未治疗样本（n = 28）和接受非烷化剂治疗样本（n = 25）的 S/T 值 Z 分数在参考均值的±3 个标准差内相当，但与接受烷化剂治疗的样本（n = 22）和克氏综合征患者（n = 8）的样本有显著差异。S/T 的 Z 分数与烷基化剂累积暴露量呈负相关（r = -0.7020）。
S/T 的 Z 分数值可量化遗传和癌症治疗对为生育保护而储存的睾丸组织的影响，有助于为患者咨询提供依据。
