Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia.
Riyadh Poisons Control & Medical Forensic Chemistry Center, Kaab Ibn Al Harith, Ar Rawdah, Riyadh, Saudi Arabia; Egyptian Drug Authority, National Organizations for Drug Control and Research (NODCAR), Giza, Egypt.
Indian J Pharmacol. 2021 Jan-Feb;53(1):13-18. doi: 10.4103/ijp.IJP_668_19.
This study was aimed to determine in vitro human whole blood-to-plasma ratio (K/) of THJ-018 by gas chromatography/mass spectrometry (GC/MS).
The samples (human blood) were sprayed with THJ-018 and an internal standard and extracted using solid-phase extraction. THJ-018 was determined in the final extracts by GC/MS.
The value for K/ was 1.56 (1.38-1.81), and red blood cell partitioning was 1.01 (1.01-1.02). The distribution of THJ-018 between whole blood and plasma was observed to be affected by temperature.
The data analysis supports the proposition that the ratio of the plasma to whole blood concentrations (1.56) is a suitable parameter characterizing THJ-018 distribution in whole blood. For toxicological analysis, it would be best to refrain from converting any drug concentration measured in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations should be determined for the individual specimens collected.
本研究旨在通过气相色谱/质谱法（GC/MS）测定 THJ-018 在人全血-血浆比（Kp）。
向含有 THJ-018 和内标的人血样本中喷洒样品，然后使用固相萃取进行提取。最后用 GC/MS 测定最终提取物中的 THJ-018。
Kp 值为 1.56（1.38-1.81），红细胞分配系数为 1.01（1.01-1.02）。THJ-018 在全血和血浆之间的分布受温度影响。
数据分析支持这样的观点，即血浆与全血浓度之比（1.56）是描述 THJ-018 在全血中分布的合适参数。对于毒理学分析，最好不要将全血中测量的任何药物浓度转换为预期的血浆或血清浓度；然而，应针对采集的个体标本确定毒性和治疗浓度。
