Mycelial compatibility, anastomosis, and nucleus numbers of eight Mexican strains isolated from .

BACKGROUND

Among entomopathogenic fungi, has been recognized as potential biocontrol agent against the Asian citrus psyllid (Hemiptera: Liviidae). Nevertheless, this fungus is poorly characterized. Previous molecular studies have shown high sequence similarities among strains, but significant differences in virulence.

OBJECTIVE

The aim of the present study was to determine mycelial compatibility and anastomosis, and nucleus numbers in mycelium and conidia of eight strains isolated from mycosed adults collected from several Mexican states.

METHODS

Mycelial compatibility and anastomosis evaluation was performed after pairing strains, leading to 36 confrontations, and cultured in chlorate minimum medium to obtain mutants for vegetative compatibility group.

RESULTS

Hypha or conidia nuclei were visualized with safranin-O and 3% KOH, and 0.05% trypan blue-lactophenol solution. strains showed compatibly and anastomosis events after confrontation. In addition, they showed one nucleus per conidium and mycelium section. It was not possible to obtain mutants from the chlorate concentrations tested.

CONCLUSIONS

To date, this is the first report demonstrating mycelial compatibility, anastomosis occurrence, and hyphae and conidia nuclei number among strains.