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从……分离出的八个墨西哥菌株的菌丝体相容性、吻合情况及细胞核数量

Mycelial compatibility, anastomosis, and nucleus numbers of eight Mexican strains isolated from .

作者信息

Pérez-González Orquídea, Gomez-Flores Ricardo, Tamez-Guerra Patricia

机构信息

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo Leon, Mexico.

出版信息

PeerJ. 2021 Apr 19;9:e11080. doi: 10.7717/peerj.11080. eCollection 2021.

DOI:10.7717/peerj.11080
PMID:33976961
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8061572/
Abstract

BACKGROUND

Among entomopathogenic fungi, has been recognized as potential biocontrol agent against the Asian citrus psyllid (Hemiptera: Liviidae). Nevertheless, this fungus is poorly characterized. Previous molecular studies have shown high sequence similarities among strains, but significant differences in virulence.

OBJECTIVE

The aim of the present study was to determine mycelial compatibility and anastomosis, and nucleus numbers in mycelium and conidia of eight strains isolated from mycosed adults collected from several Mexican states.

METHODS

Mycelial compatibility and anastomosis evaluation was performed after pairing strains, leading to 36 confrontations, and cultured in chlorate minimum medium to obtain mutants for vegetative compatibility group.

RESULTS

Hypha or conidia nuclei were visualized with safranin-O and 3% KOH, and 0.05% trypan blue-lactophenol solution. strains showed compatibly and anastomosis events after confrontation. In addition, they showed one nucleus per conidium and mycelium section. It was not possible to obtain mutants from the chlorate concentrations tested.

CONCLUSIONS

To date, this is the first report demonstrating mycelial compatibility, anastomosis occurrence, and hyphae and conidia nuclei number among strains.

摘要

背景

在昆虫病原真菌中，已被认为是针对亚洲柑橘木虱（半翅目：木虱科）的潜在生物防治剂。然而，这种真菌的特征描述很少。先前的分子研究表明，菌株之间存在高度的序列相似性，但毒力存在显著差异。

目的

本研究的目的是确定从墨西哥几个州采集的感染真菌的成虫中分离出的8株菌株的菌丝体相容性和吻合情况，以及菌丝体和分生孢子中的细胞核数量。

方法

在菌株配对后进行菌丝体相容性和吻合评估，共进行36次对峙，并在氯酸盐基本培养基中培养以获得营养体相容性组的突变体。

结果

用番红O和3%氢氧化钾以及0.05%台盼蓝-乳酸酚溶液观察菌丝或分生孢子细胞核。菌株在对峙后表现出相容性和吻合事件。此外，它们在每个分生孢子和菌丝体切片中显示一个细胞核。在所测试的氯酸盐浓度下无法获得突变体。

结论

迄今为止，这是第一份证明菌株之间菌丝体相容性、吻合现象以及菌丝和分生孢子细胞核数量的报告。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/1ea79a9699b1/peerj-09-11080-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/b73ffc8a0e79/peerj-09-11080-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/daefbcfb7bc0/peerj-09-11080-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/ae53d8d3e028/peerj-09-11080-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/2c0baa71421f/peerj-09-11080-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/1ea79a9699b1/peerj-09-11080-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/b73ffc8a0e79/peerj-09-11080-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/daefbcfb7bc0/peerj-09-11080-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/ae53d8d3e028/peerj-09-11080-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/2c0baa71421f/peerj-09-11080-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6848/8061572/1ea79a9699b1/peerj-09-11080-g005.jpg

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