Jacobson W, South M, Hughes G, Davies R, Morley C
Department of Paediatrics, University of Cambridge, Addenbrooke's Hospital, United Kingdom.
J Pediatr. 1988 Aug;113(2):368-72. doi: 10.1016/s0022-3476(88)80286-5.
A technique for detecting the presence of pulmonary surfactant in the tracheal aspirates obtained from preterm babies is described. The specimens were examined by means of polarized light microscopy. Surfactant could be simply and rapidly identified by its appearance as birefringent particles in volumes of aspirate as little as 1 microliter. Tracheal aspirate specimens from 108 babies, obtained on the first day of life, were examined without knowledge of the patient's clinical details. When the samples from each baby were subdivided into three groups on the basis of the amount of surfactant particles seen, this subgrouping corresponded well with the babies' ventilatory requirements at the time of sample collection. This method of detecting surfactant material may prove valuable in determining the degree of surfactant deficiency in individual preterm babies with respiratory illness.
本文描述了一种检测从早产儿获得的气管吸出物中肺表面活性物质存在的技术。通过偏光显微镜对标本进行检查。表面活性物质可以通过其在低至1微升吸出物中呈现为双折射颗粒的外观而被简单快速地识别。在不知道患者临床细节的情况下，对108名婴儿出生第一天获得的气管吸出物标本进行了检查。当根据所见表面活性物质颗粒的数量将每个婴儿的样本分为三组时，这种亚组划分与样本采集时婴儿的通气需求非常吻合。这种检测表面活性物质材料的方法可能在确定患有呼吸系统疾病的个体早产儿的表面活性物质缺乏程度方面具有价值。
